高效液相色谱法测定舒肝丸中木香及厚朴的4个特征成分

Simultaneous determination of 4 active ingredients in Aucklandiae Radix and Magnoliae Officinalis Cortex in Shugan pills by HPLC

目的:建立高效液相色谱法同时测定舒肝丸中去氢木香内酯、木香羟内酯、和厚朴酚与厚朴酚4个成分的含量。方法:采用Acclaim C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.05%磷酸(47∶53)为流动相,流速1.0 m L·min^(-1),检测波长为230 nm。结果:和厚朴酚、木香羟内酯、去氢木香内酯、厚朴酚进样量分别在0.020~0.401、0.025~0.499、0.051~1.029、0.040~0.796μg范围内线性关系良好,相关系数均为0.999 9;平均回收率(n=9)分别为99.1%、100.4%、100.8%、101.4%,RSD分别为1.4%、1.7%、1.7%、2.0%。10批样品中和厚朴酚、木香羟内酯、去氢木香内酯及厚朴酚的含量范围分别为0.058~0.400、0.105~0.280、0.200~0.568、0.229~0.816 mg·g^(-1)。结论:该方法为舒肝丸的质量评价提供了更为全面的科学依据。

Objective： To establish an HPLC method for simultaneous determination of 4 active ingredients （ dehydroeostuslactone, costunolide, honokiol, magnolol ） in Shugan pills. Methods： The chromalographic separation was performed on an Acclaim C18 （ 250 mm×4.6 mm, 5 μm ） column with acetonitrile -0.05% phosphoric acid solution （ 47 ： 53 ） as mobile phase. The flow rate was 1.0 mL· min^-1. The detection wavelength was set at 230 nm. Results： The calibration curves were linear within the range of 0.020-0.400 μg for honokiol, 0.024-0.498 μg for costunolide, 0.051-1.029 μg for dehydroeostuslactone and 0.039-0.795 p.g for magnolol. All ingredients showed a good linear relationship （ r=0.999 9 ）. The average recoveries （ n=9 ）were 99.1%, 100.4%, 100.8% and 101.4%, respectively with RSDs of 1.4%, 1.7%, 1.7% and 2.0%, respectively. The contents of honokiol, costunolidein, dehydrocostuslactone and magnolol in 10 samples were 0.058-0.400 mg·g^-1 , 0.105- 0.280 mg·g^-1 , 0.200-0.568 and 0.229-0.816 mg·g^-1 , respectively. Conclusion： The method provided a more comprehensive scientific basis for the quality evaluation of Shugan pills.