羌活中异欧前胡素在大鼠各肠段的吸收特点

Absorption Characteristics of Isoimperatorin from Notopterygium in Different Parts of Rat's Intestines

目的探讨羌活中异欧前胡素在大鼠肠段的吸收特性。方法建立大鼠体内单向肠灌流模型,以酚红为标示物,采用反相高效液相色谱法分别测定羌活粗提物中异欧前胡素的含量,并观察其与对照品在大鼠的肠内吸收情况。结果羌活粗提物中异欧前胡素含量为(0.43±0.02)%(n=5),其在大鼠十二指肠、空肠、回肠、结肠的有效渗透系数(Peff)分别为(5.28±0.82),(4.47±0.56),(4.17±0.94),(4.32±0.68)×10-5cm·s-1,差异无统计学意义(P>0.05);与对照品比较,羌活粗提物中异欧前胡素在各肠段吸收较佳。结论羌活粗提物中异欧前胡素在肠内具有较好的吸收特性,该研究结果可为口服羌活剂型设计提供理论依据。

Objective To study the absorption features of isoimperatorin in intestine of rat. Methods Establish a single-pass intestinal perfusion model of rat, take phenolsulfonphthalein as a marker for the detection of isoimperatorin concentration from crude extracts of notopterygium,and observe the absorption features of isoimperatorin and its reference substance in intestine of rat. Results The content of isoimperatorin in crude extracts was( 0. 43 ± 0. 02) %( n = 5). The absorption parameters of duodenum,jejunum,ileum,colon were( 5.28±0.82),( 4.47± 0.56),( 4.17± 0.94),( 4.32± 0.68) ×10-5cm·s-1,respectively. There were no significant differences among them( P>0.05). Compared with the reference substance of isoimperatorin,crude extracts showed better absorption features. Conclusion Isoimperatorin from crude extracts have better characteristics of absorption. This study can provide theoretical basis for design of notopterygium oral formulation.