一测多评法测定重楼药材中4种皂苷类成分含量

Determination of Four Saponin Components in Paridis Rhizhma by Quantitative Analysis of Multi-components with Single-marker

目的建立一测多评法测定重楼药材中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量。方法采用高效液相色谱法,Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水为流动相梯度洗脱,检测波长203 nm,流速1.0 m L·min^(-1),柱温25℃;以重楼皂苷Ⅶ为内参物,建立其与去重楼皂苷Ⅰ、Ⅱ、Ⅵ的相对校正因子,采用一测多评法和标准曲线法同时计算皂苷含量,并进行比较。结果重楼药材中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量分别为0~1.120%,0~0.421%,0~0.264%,0.109%~0.485%,一测多评法测定的结果与标准曲线法无明显差异(RSD<2.0%)。结论一测多评法操作简单,重复性好,可为重楼的质量标准修订提供参考。

Objective To establish a quality evaluation method for determination of paris saponin Ⅰ,Ⅱ,Ⅵ,Ⅶ in paridis rhizhma by quantitative analysis of multi-components with single-marker( QAMS). Methods An HPLC method and a Phenomenex Luna C18column( 4.6 mm×250 mm,5 μm) were used. The mobile phase was acetonitrile-water( 48∶52) at a flow rate of 1.0 m L·min-1. The detection wavelength was 203 nm and column temperature was 25 ℃. Parissaponin Ⅶ was used as the internal reference substance. The relative correlation factors of parissaponin Ⅰ,Ⅱ,Ⅵ were calculated by standard curve method and QAMS. Results The QAMS method could be used for determination of four saponin components at the same time without significant difference as compared with the results of standard curve method( RSD < 2. 0%). Conclusion QAMS method is simple and reproducible,which can provide a reference for quality standard revision for paridis rhizhma.