摘要
将化工本科实验转型为综合性设计实验,对于提高学生的实验能力、培养学生发现问题和解决问题的能力起着非常重要的作用。讨论了将实验"丹皮酚的提取和分离"调整为综合设计性实验的内容和过程,包括采用水蒸汽蒸馏法提取丹皮酚粗品,经过重结晶制得丹皮酚纯品,并进行含量测定和结构鉴定;然后再以提取丹皮酚后的残渣为原料,加入乙醇提取其中的单萜苷类化合物,再采用大孔吸附树脂富集单萜苷类化合物制备得到总单萜苷样品,最后用HPLC分析主要单萜苷类化合物的含量。通过实验,学生能够掌握综合实验技能,激发对科学研究的兴趣。
The comprehensive and designed experiment of undergraduate practicals for the chemical engi-neering of natural products could significantly increase the students, experimental abilities,train them to be able to seek and solve problems. The article discuss the contents and procedures of designed comprehensive practical derived from “the extraction and separation of the paeonol”. 1 t includes： extraction of crude pae-onol using steam distillation; production of pure paeonol using recrystallization; measurement of contents and identification of the structure; ethanol isolation of monoterpene glycosides from residues after paeonol extraction; production of total monoterpene glycoside using macroporous resin to enrich monoterpene glyco- sides; finally analysis of contents of the main monoterpene glycosides using HPLC. Through the experiment, students can improve their innovatory ability and their researching interesting.
出处
《实验室科学》
2017年第4期10-15,共6页
Laboratory Science
基金
河南省教育技术装备和实践教育研究(项目编号:GZS357)
河南科技大学研究生教改项目(项目编号:2015YJG-025)
