高效液相色谱法测定银龙清热口服液中绿原酸的含量

Content Determination of Chlorogenic Acid in Yinlongqingre Oral Solution by HPLC

目的建立高效液相色谱(high performance liquid chromatography,HPLC)法测定银龙清热口服液中绿原酸含量的方法。方法色谱柱为Diamonsil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇∶水∶冰醋酸(20∶80∶1);检测波长为324 nm;流速为1.0 ml/min;柱温为35℃。结果在进样量为10μl时,绿原酸浓度在10.0202~125.2525μg/ml,其浓度与峰面积积分值呈良好的线性关系,以绿原酸质量浓度为横坐标X(μg/ml)、峰面积积分值为纵坐标Y进行线性回归,得回归方程为Y=29234X-3971.5,R^2=0.9994;平均加样回收率(n=9)为100.41%,相对标准偏差(relative standard deviation,RSD)为2.70%。结论 HPLC法测定银龙清热口服液中绿原酸的含量结果准确、稳定、重现性好,且简便易行,可用于该制剂中绿原酸的含量测定。

Objective To establish a method for the content determination of chlorogenic acid in Yinlongqingre oral solution by high performance liquid chromatography （HPLC）. Methods The determination was performed with a Dia- monsil C18 column （250 mm×4. 6 mm, 5 μm） and a mobile phase consisting of CH3 OH-H20-CH3 COOH （20 ： 80 ： 1 ）. The detective wavelength was 324 rum, the flow rate was 1.0 ml/min and the column temperature was maintained at 35 ℃. Results The calibration curve of chlorogenic acid was linear over the range of 10. 0202 - 125. 2525 ~g/ml, with the regression equation of Y = 29234X- 3971.5, R2 = 0. 9994. The average recovery rate was 100. 41% （n = 9）, and relative standard deviation （RSD） was 2. 70%. Conclusion The HPLC method is simple, accurate and reproducible to determi- nate the content of Chlorogenic acid in Yinlongqingre oral solution.