大麻哈鱼硫酸软骨素及其酸降解产物的抗氧化活性

Antioxidant activities of chondroitin sulfate and its acid degradation products of Oncorhynchus keta

目的以大麻哈鱼鼻软骨为研究对象,制备硫酸软骨素粗提物、醇沉产物和酸降解产物,对比分析其体外抗氧化活性。方法通过测定硫酸软骨素粗提物、醇沉产物和酸降解产物对DPPH·、·OH和O_2^-·自由基的清除特性,比较其抗氧化能力。结果醇沉产物对DPPH·没有明显的清除作用,粗提物和酸降解物产物对DPPH·有一定的清除作用,但后者的清除能力远大于前者。粗提物、醇沉产物和酸降解产物对·OH自由基均有明显的清除作用;粗提物和醇沉产物对O_2^-·自由基均无清除作用,但酸降解产物对O_2^-·自由基有较强的清除作用。酸降解产物对3种自由基的清除能力均较强,IC50值均小于1.5 mg/m L。结论大麻哈鱼鼻软骨硫酸软骨素酸降解产物的抗氧化活性最强。

Objective Using Oncorhynchu sketa nasal cartilage as research object, the crude chondroitin sulfate extracts, ethanol precipitated chondroitin sulfate and acid degradation products were prepared, and their antioxidant activities in vitro were compared and analyzed. Methods The scavenging capacities on three kinds of free radicals including DPPH·, ·OH and O2～-· of the crude chondroitin sulfate extracts, ethanol precipitated chondroitin sulfate and acid degradation products were measured, so as to compare their antioxidant activities. Results The ethanol precipitated chondroitin sulfate had no obvious scavenging effect on DPPH·, while the crude chondroitin sulfate extracts and acid degradation products had certain scavenging effects on DPPH·, and the scavenging ability of acid degradation products was much greater than that of crude chondroitin sulfate extracts. The crude chondroitin sulfate extracts, ethanol precipitated chondroitin sulfate, and acid degradation products of chondroitin sulfate all had obvious scavenging effects on ·OH. The crude chondroitin sulfate extracts and ethanol precipitated chondroitin sulfate had no scavenging effects on O2^-·, but acid degradation products of chondroitin sulfate had a strong scavenging effect on O2^-·. The scavenging capacities of acid degradation products of chondroitin sulfate to DPPH·,·OH and O2^-· was the best, and their IC50 values were less than 1.5 mg/m L. Conclusion The antioxidant activity of acid degradation products of chondroitin sulfate from Oncorhynchus keta nasal cartilage is the best.