高效液相色谱-三重四级杆质谱联用法检测荷斯坦和水牛原料乳中磺胺类药物残留

Determination of sulfonamides residues in raw Holstein and buffalo milk by high performance liquid chromatography-tandem mass spectrometry

目的分析目前我国南方地区荷斯坦原料乳和水牛原料乳中磺胺类药物的残留情况。方法采集浙江和广西地区荷斯坦牛牧场和水牛牧场的120份原料乳,采用高效液相色谱-三重四级杆质谱联用法检测其中的磺胺类药物残留。结果方法的检出限和回收率分别为0.05~0.10μg/L和73.2%~108.3%。水牛乳中磺胺类药物的残留量低于6.67μg/L,荷斯坦牛乳中磺胺类药物的残留量低于7.88μg/L。结论高效液相色谱-三重四级杆质谱联用法检测牛乳中磺胺类药物残留的结果准确可靠,我国南方地区的荷斯坦原料乳和水牛原料乳总体上处于安全范围。

Objective To analyze the sulfonamides residues in raw milk of Holstein and buffalo in south China. Methods A total of 120 milk samples were collected from Holstein and buffalo farms in Zhejiang and Guangxi provinces, and the sulfonamides residues in raw milk were determined by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. Results The limits of determination（LODs） and recoveries of HPLC-MS/MS were 0.05～0.10 μg/L and 73.2%～108.3%%, respectively, and the residue amounts of sulfonamides in raw milk of Holstein and buffalo were lower than 6.67 μg/L and 7.88 μg/L. Conclusion HPLC-MS/MS is an accurate and reliable method for sulfonamides residues measurement in milk, and the sulfonamides residues in raw Holstein and buffalo in south China are in safety situation.