大肠杆菌O157快速检测板在肉与肉制品检测中的应用

Application of rapid petri-dish in the detection of Escherichia coli O157 in meat and meat products

目的研究大肠杆菌O157快速检测板在肉与肉制品检测中的应用。方法以GB/T 4789.36-2008《食品卫生微生物学检验大肠埃希氏菌O157:H7/NM检验》作为参考方法,研究大肠杆菌O157快速检测板在肉与肉制品检测中应用的灵敏度、特异性、假阳性率、假阴性率、准确度等指标,同时利用卡方检验分析阳性率显著性差异。结果大肠杆菌O157快速检测板灵敏度为100%,不存在假阴性,特异性为96.4%,假阳性率为3.6%,准确度为98%,显著性差异卡方值卡Χ~2为0,均达到定性方法验证的相关指标。结论大肠杆菌O157检测板技术具有快速准确、灵敏度高、操作简单的特点,可以作为一种快速筛查方法广泛应用于肉与肉制品大肠杆菌O157的检测。

Objective To evaluate the application value of rapid petri-dish in the detection of Escherichia coli O157 in meat and meat products. Methods The sensitivity, specificity, false positive rate, false negative rate and accuracy of E. coli O157 rapid petri-dish in detection of meat and meat products were investigated by using national standard GB/T 4789.36-2008 Microbiological examination of food hygiene examination E. coli O157：H7/NM. Meanwhile, the significant difference of positive rates was analyzed by the chi-square. Result The sensitivity of E. coli O157 petri-dish was 100%, the false negative rate was 0, the specificity was 96.4%, the false positive rate was 3.6%, the accuracy rate was 98%, and the chi-square（Χ^2） was 0. The results showed that all parameters conformed to the requirements of qualitative methods validation. Conclusion It is feasible to detect E. coli O157 by the rapid petri-dish as a rapid screening method for its speediness, accuracy, high sensitivity and easy operation.