针灸干预对溃疡性结肠炎动物肠黏膜炎症反应、机体免疫应答平衡的影响

Effect of acupuncture intervention on the intestinal mucosal inflammatory response and immune response balance in animals with ulcerative colitis

目的:研究针灸干预对溃疡性结肠炎(UC)动物肠黏膜炎症反应、机体免疫应答平衡的影响。方法:选择成年、雄性、SPF级别的SD大鼠并随机分为对照组、UC组和针灸组,建立UC动物模型后给予针灸干预。干预后14天时,检测肠黏膜中炎症介质及Th1/Th2/Th17/Treg细胞因子的表达量、血清中炎症介质及Th1/Th2/Th17/Treg细胞因子的含量。结果:UC组大鼠肠黏膜中NF-kB、HMGB-1、TNF-α、IL-1β、IFN-γ、IL-17的mRNA表达量及血清中HMGB-1、TNF-α、IL-1β、IFN-γ、IL-17的含量显著高于对照组,肠黏膜中IL-4、IL-5、TGF-β1的mRNA表达量及血清中IL-4、IL-5、TGF-β1的含量均显著低于对照组;针灸组大鼠肠黏膜中NF-kB、HMGB-1、TNF-α、IL-1β、IFN-γ、IL-17的mRNA表达量及血清中HMGB-1、TNF-α、IL-1β、IFN-γ、IL-17的含量显著低于UC组,肠黏膜中IL-4、IL-5、TGF-β1的mRNA表达量及血清中IL-4、IL-5、TGF-β1的含量均显著高于UC组。结论:针刺干预对溃疡性结肠炎动物肠黏膜炎症反应和免疫应答均具有调节作用。

Objective：To study the effect of acupuncture intervention on the intestinal mucosal inflammatory response and immune response balance in animals with ulcerative colitis（UC）.Methods：Adult male SPF SD rats were selected and randomly divided into the control group,UC group and acupuncture group,and then the acupuncture intervention was established after the UC animal model was established.Fourteen days after intervention,the expression of inflammatory mediators and Th1/Th2/Th17/Treg cytokines in intestinal mucosa,and the levels of inflammatory mediators and Th1/Th2/Th17/Treg cytokines in serum were detected.Results：NF-kB,HMGB-1,TNF-α,IL-1β,IFN-γand IL-17 mRNA expression in intestinal mucosa as well as HMGB-1,TNF-α,IL-1β,IFN-γand IL-17 levels in serum of UC group were significantly higher than those of control group while IL-4,IL-5and TGF-β1mRNA expression in intestinal mucosa as well as IL-4,IL-5and TGF-β1levels in serum were significantly lower than those of control group;NF-kB,HMGB-1,TNF-α,IL-1β,IFN-γand IL-17 mRNA expression in intestinal mucosa as well as HMGB-1,TNF-α,IL-1β,IFN-γand IL-17 levels in serum of acupuncture group were significantly lower than those of UC group while IL-4,IL-5and TGF-β1mRNA expression in intestinal mucosa as well as IL-4,IL-5and TGF-β1levels in serum were significantly higher than those of UC group.Conclusion：Acupuncture intervention can regulate the intestinal mucosal inflammatory response and immune response of animals with ulcerative colitis.