雌孕激素受体ER、PR表达与子宫内膜癌病灶生长的相关性研究

Correlation of estradiol and progesterone receptors ER and PR expression with the growth of endometrial cancer

目的:探讨雌孕激素受体ER、PR表达与子宫内膜癌病灶生长的相关性。方法:收集接受治疗的子宫内膜癌患者80例,取子宫内膜癌组织及癌旁正常组织,采用免疫组化法检测其中ER、PR阳性表达情况,采用荧光定量PCR测定其中增殖、凋亡基因mRNA表达量。结果:肿瘤组织中ER、PR阳性表达量均显著低于癌旁组织(P<0.01);ER阳性组、PR阳性组肿瘤组织中增殖基因KCC1、RRM2、SRPX2、Snail mRNA的表达量分别低于ER阴性组、PR阴性组;抗凋亡基因Wip-1、Bcl-2mRNA的表达量分别低于ER阴性组、PR阴性组,促凋亡基因Bid、Bax、Fas mRNA的表达量分别高于ER阴性组、PR阴性组(P<0.01)。结论:子宫内膜雌孕激素受体ER、PR阳性表达者,肿瘤增殖活性较低、凋亡活性较高,恶性程度低于ER、PR阴性表达者。

Objective：To study the correlation between estradiol and progesterone receptors ER and PR expression and the growth of endometrial cancer.Methods：A total of 80 patients with endometrial cancer who were treated in the Fourth People＇s Hospital of Shaanxi and the First Affiliated Hospital of Xi＇an Jiaotong University between January 2013 and January 2017 were collected,endometrial cancer tissue and para-carcinoma normal tissue were collected,immunohistochemical method was used to detect positive expression of ER and PR,and fluorescence quantitative PCR was used to detect the mRNA expression of proliferation and apoptosis genes.Results：The positive expression levels of ER and PR in tumor tissue were significantly lower than those in para-carcinoma tissue（P〈0.01）;proliferation genes KCC1,RRM2,SRPX2 and Snail mRNA expression levels in ER-positive group and PR-positive group of tumor tissue were lower than those in ER-negative group and PR-negative group;anti-apoptosis genes Wip-1and Bcl-2 mRNA expression were lower than those in ER-negative group and PR-negative group respectively while pro-apoptosis genes Bid,Bax and Fas mRNA expression were higher than those in ER-negative group and PR-negative group respectively（P〈0.01）.Conclusion：In patients with positive expression of endometrial estradiol and progesterone receptors ER and PR,tumor proliferation activity was lower while apoptosis activity was more active indicating lower malignancy degree than patients with negative ER and PR expression.