癫痫患者血清Hcy代谢与神经细胞因子、凋亡分子含量的相关性

Correlation of serum Hcy metabolism with nerve cytokine and apoptosis molecule levels in patients with epilepsy

目的:研究癫痫患者血清同型半胱氨酸(Hcy)代谢与神经细胞因子、凋亡分子含量的相关性。方法:选择在我院就诊的癫痫发作患者和癫痫非发作患者分别作为癫痫发作组和癫痫非发作组,另取同期健康志愿者作为对照组。采集血清并测定Hcy代谢指标、神经细胞因子、凋亡分子的含量。结果:癫痫发作组患者血清中Hcy、BDNF、NGF、NSE、IGF-I、Bax、Caspase-3、MDA、8-OHdG的含量显著高于癫痫非发作组及对照组,VitB12、Fol、Bcl-2的含量显著低于癫痫非发作组及对照组;癫痫非发作组患者血清中Hcy、VitB12、Fol、BDNF、NGF、NSE、IGF-I、Bcl-2、Bax、Caspase-3、MDA、8-OHdG的含量与对照组比较无显著性差异;癫痫发作患者血清中Hcy含量与BDNF、NGF、NSE、IGF-I、Bax、Caspase-3、MDA、8-OHdG含量呈正相关,与Bcl-2含量呈负相关,VitB12、Fol含量与BDNF、NGF、NSE、IGF-I、Bax、Caspase-3、MDA、8-OHdG含量呈负相关,与Bcl-2含量呈正相关。结论:癫痫发作患者血清Hcy代谢异常能够加重神经元损伤及神经元凋亡。

Objective：To study the correlation of serum homocysteine（Hcy）metabolism with nerve cytokine and apoptosis molecule levels in patients with epilepsy.〗Methods：Patients with epileptic seizures and non-epileptic seizures who were treated in our hospital between January 2014 and December 2016 were selected as epileptic seizure group and non-epileptic seizure group respectively,and healthy volunteers during the same period were selected as control group.The serum was collected to determine the levels of Hcy metabolism indexes,nerve cytokines and apoptosis molecules.Results：Serum Hcy,BDNF,NGF,NSE,IGF-I,Bax,Caspase-3,MDA and 8-OHdG levels of epileptic seizure group were significantly higher than those of non-epileptic seizure group and control group while VitB12,Fol and Bcl-2levels were significantly lower than those of nonepileptic seizure group and control group;serum Hcy,VitB12,Fol,BDNF,NGF,NSE,IGF-I,Bcl-2,Bax,Caspase-3,MDA and 8-OHdG levels of non-epileptic seizure group were not significantly different from those of control group;serum Hcy level in patients with epileptic seizure was positively correlated with BDNF,NGF,NSE,IGF-I,Bax,Caspase-3,MDA and 8-OHdG levels,and negatively correlated with Bcl-2level while VitB12 and Fol levels were negatively correlated with BDNF,NGF,NSE,IGF-I,Bax,Caspase-3,MDA and 8-OHdG levels,and positively correlated with Bcl-2level.Conclusion：Abnormal serum Hcy metabolism in patients with epileptic seizures can aggravate the neuron injury and apoptosis.