水稻无蜡质叶片突变体wcl1的图位克隆

Mapping-based Cloning of a Wax Crystal-Sparse Leaf Mutant wcl1 in Rice

从水稻T-DNA插入突变体库中筛选获得1份叶片表皮无蜡质的突变体wcl1(Wax Crystal-Sparse Leaf 1),突变体wcl1有如下主要特征:一是突变体叶片角质层蜡质减少,二是突变体的干旱敏感性高于野生型。通过图位克隆技术,克隆了wcl1基因,其编码酮酯酰辅酶A(LOC_OS09g25850),在突变体中LOC_OS09g25850基因的第10个外显子处鸟嘌呤(G)突变为胸腺嘧啶(T)导致转录提前终止,经分析表明突变体wcl1是一个wsl2基因的等位突变体。

Here a wax crystal-sparse leaf mutant（wcl1）was screened from rice T-DNA Insertion Mutant Library. The mutant wcl1 hastwo main features as following ：1）the wcl1 leaf cuticular wax reduced;2）the drought sensitivity in the wcl1 plant was higher than wild type.Map-based cloning of wcl1 revealed that a point mutation of G to T in the 10 th exonof gene LOC_OS09g25850encodingketoacyl coenzyme Aresulted in the termination of transcription. The analysisshowed that the wcl1 was allelic to wsl2.