HPLC法同时测定补骨脂药材中补骨脂二氢黄酮和补骨脂酚的含量

Simultaneous Determination of Bavachin and Bakuchiol in Psorslea corylifolia by HPLC

目的:建立同时测定补骨脂药材中补骨脂二氢黄酮和补骨脂酚含量的方法。方法:采用高效液相色谱法。色谱柱为Shim-Pack VP-ODS,流动相为乙腈-水(梯度洗脱),流速为1.0 mL/min,检测波长为246 nm,柱温为30℃,进样量为10μL。结果:补骨脂二氢黄酮、补骨脂酚检测进样量线性范围分别为48.9～489 ng(r=0.999 8)、784～7 840 ng(r=0.999 9);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为95.30%～99.63%(RSD=1.45%,n=9)、96.89%～100.82%(RSD=1.36%,n=9)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于补骨脂药材中补骨脂二氢黄酮和补骨脂酚含量的同时测定;不同辐照剂量下药材样品中待测成分的含量均无明显变化。

OBJECTIVE： To establish a method for content determination of bavachin and bakuchiol in Psoralea corylifolia. METHODS： HPLC method was adopted. The determination was performed on Shim-Pack VP-ODS column with mobile phase con- sisted of acetonitrile-water （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 246 nm, the col- umn temperature was 30 ℃ , and the sample size was 10 μL. RESULTS： The linear ranges of bavachin and bakuchiol were 48.9-489 ng（r=0.999 8）, 784-7 840 ng（r=0.999 9）. RSDs of precision, stability and reproducibility tests were all lower than 2.0%. Average recoveries ranged 95.30%-99.63% （RSD=l.45%, n----9）, 96.89%-100.82% （RSD=1.36%, n=9）. CONCLU- SIONS： The method is simple, accurate, stable and reproducible, and can be used for the simultaneous determination of bavachin and bakuchiol in P. corylifolia.