射干传统功效考证及其实验药理学验证

Textual Research on Traditional Effect Verification of Belamcanda chinensis and Its Pharmacological Experimental Verification

目的:通过古籍文献及现代文献,了解射干从古至今的功效演变及治疗病种变化,利用药理药效实验手段,对其主要功效进行验证,将传统中药特色优势与现代化科学技术相结合,有助于推进中医药现代化进程,对中药新药研发有效成分、治疗病种的选择具有重要意义。方法:从本草考证射干功能主治、从医籍考证射干治疗病症、基于《普济方》数据库管理系统分析射干复方治疗病种,对射干主要功效主治进行总结,结合现代药理研究射干主要成分抗炎作用机制,筛选动物模型和考查指标,对射干功效进行初步验证。结果:综合古典本草医籍应用情况和方药数据库分析结果表明射干有消痰、利咽的功效,是治疗咽痛喉痹的要药;现代研究中实验组血清及咽喉组织中IL-4、血清及肺组织中IgE、血清中LTC4水平均较模型组显著降低(P<0.05)。结论:射干提取物对慢性咽炎的治疗作用可能是通过降低血清及肺组织中IgE水平,抑制血清及咽喉组织中IL-4和血清中LTC4的表达等来实现。

Through reviewing ancient and modern literatures, the effect evolution and disease treatment changes of Belamcanda chinensis were understood. The pharmacological experiments were used to verify its main effects. The combination of featured advantages of traditional Chinese medicine（TCM） and modern science and technology contributed to the promotion of TCM modernization. It had important significance for the development of effective components, selection of disease types in the treatment for research and development of new TCM drugs. The indication of Belamcanda chinensis was verified from textual research. The treatment of disease types by Belamcanda chinensis was verified from medical books. The treatment of disease types by Belamcanda chinensis compound was analyzed based on the Pujifang database management system. Main indications of Belamcanda chinensis were summarized. Modern pharmacological studies on anti-inflammatory mechanism of main components of Belamcanda chinensis were combined to screen animal models and investigation indexes for the preliminary verification of the efficacy of Belamcanda chinensis.The comprehensive application of classical herbal medicine books and prescription database analysis results showed that removing phlegm and relieving sore throat were the efficacy of Belamcanda chinensis, which was an important medicine in the treatment of pharyngitis and sore throat. In the modern research, serum of experimental group, IL-4 in throat tissues, as well as IgE and LTC4 level in serum and lung tissues were significantly reduced compared to the model group（P〈0.05）. It was concluded that the treatment effect of Belamcanda chinensis extract to chronic pharyngitis may be through the decreasing of IgE level in serum and lung tissues, inhibiting IL-4 expression in serum and throat tissues, and the LTC4 expression in serum.