白木香AsMAPK3基因的克隆及表达分析

Cloning and Expression Analysis of AsMAPK3 from Aquilaria sinensis( Lour. ) Gilg

目的:克隆白木香中分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)基因,检测其组织表达及诱导表达特性。方法:在454高通量测序获得部分c DNA序列基础上,利用RACE方法获得全长cDNA。采用NCBI在线Blastp、DNAman和MEGA4软件,对序列进行生物信息学分析。随后,通过荧光定量PCR检测目的基因的组织表达及诱导表达特性。结果:扩增到了白木香中分裂原活化蛋白激酶基因AsMAPK3,并对其进行了生物信息学分析和表达特性分析。结论:通过As MAPK3的克隆及分析,为后续验证其生物学功能奠定了基础。

Objective：To clone a mitogen-activated protein kinase （MAPK） gene from Aquilaria sinensis （Lour.） Gilg，and to analyze its expression profiles in different tissues and inducers.Methods：Rapid amplification of cDNA ends （RACE） technology was used to clone the full-length cDNA of the MAPK gene on the basis of its partial cDNA sequence obtained from our previous 454-sequenced dataset.Using NCBI online Blastp，DNAman and MEGA4，the sequences were bioinformatically analyzed.After that，its expression profiles in different tissues and inducers were analyzed by real-time PCR.Results：The MAPK gene，AsMAPK3 was cloned from A.sinensis and its expression profiles were analyzed.Conclusion：Our works on gene cloning and expression analysis provide a substantial foundation for follow-up biofunction analysis of the AsMAPK3.