正交试验法优选陈皮橙皮苷超声提取工艺

Optimizing Ultrasonic Extraction of Hesperidin from Citri Reticulatae Pericarpium Based on Orthogonal Experiments Design

目的:优选陈皮中橙皮苷的超声提取工艺。方法:通过单因素试验和正交试验对提取工艺进行优化设计,采用高效液相测定橙皮苷的含量,考察甲醇体积分数、超声时间、超声温度、液料比对橙皮苷提取量的影响。结果:影响陈皮橙皮苷提取量各因素的先后次序为甲醇体积分数>超声温度>超声时间>液料比。最佳提取工艺条件为甲醇体积分数100%,超声温度70℃,超声时间60 min,液料比80∶1。结论:优选的提取工艺稳定可行,具有方便、高效、节约成本等优点。

Objective：To optimize ultrasonic extraction of hesperidin from Citri Reticulatae Pericarpium.Methods：The content of hesperidin was determined by HPLC.The effects of methanol concentration，ultrasonic time，ultrasonic temperature and liquid-material ratio on the content of hesperidin in extracts were investigated by single factor experiments and orthogonal experiments.Results：The decreasing order of importance of the four process conditions affecting hesperidin extraction was methanol concentration，ultrasonic temperature，ultrasonic time，liquid-material ratio.The orthogonal experimental results showed that the optimal conditions were methanol concentration 100%，ultrasonic temperature 70 ℃，ultrasonic time 60 min，liquid-material ratio 80∶1.Conclusion：The optimized process is stable and reasonable，accurate and reliable.This optimum extraction process also had advantages of convenience，high efficiency and cost saving.