六种不同产地的肉桂中桂皮醛及总挥发油的含量测定

The determination of cinnamaldehyde and essential oil in Rougui collected from six habitats

目的:测定6种不同产地共18批肉桂中桂皮醛及总挥发油的含量,为肉桂的质量评价提供科学依据和方法。方法:桂皮醛的含量测定采用高效液相二极管阵列检测法(HPLC-DAD),色谱柱为CAPCELL PAK C_(18) MGⅡ S5(4.6×250mm,5μm),流动相为乙腈-水(38:62),流速为1 mL·min^(-1),柱温为35℃,检测波长290 nm,进样量10μL;总挥发油的含量测定采用水蒸气蒸馏法。结果:不同产地的肉桂中桂皮醛含量有明显差异,越南文安产含量最高,老挝产含量最低,总挥发油含量测定结果亦如此。结论:此研究结果可为肉桂的临床用药提供科学参考和依据。

Objective： Cinnamaldehyde and essential oil of 18 batches Rougui collected from six different habitats were detected to provide scientific basis and method for quality evaluation of Rougui. Method： A HPLC-DAD method was applied to the determination of cinnamaldehyde. The samples were analyzed on a CAPCELL PAK C18 MG II S5 column （4.6×250 mm, 5 μm） at 40℃ .The mobile phase was acetonitrile-water （38：62） at flow rate of 1 mL.min1. The detection wavelength was aet at 290 nm, and the sample size was 10 μL. Meanwhile, the essential oil of cinnamon was detecfed by hydrodistillation. Result： Difference were found in the content of cinnamaldehyde and essential oil among samples from different cultivation areas. Rougui from Vietnam had the highest content and Rougui from Laos had the lowest content. Conclusion： The results provid a scientific basis for clinic medicine and further study of Rougui.