摘要
目的:建立同时测定康复新液中尿苷和黄嘌呤含量的方法。方法:采用HPLC法,Diamonsil C18(250mm×4.6 mm,5μm)色谱柱,甲醇-水(5:95)作为流动相,于254 nm波长下检测,流速1.0 mL·min^(-1),柱温30℃。结果:尿苷、黄嘌呤分别在0.10~3.96μg·mL^(-1)(r^2=0.9998)、0.52~20.80μg·mL^(-1)(r^2=0.9992)浓度范围内线性关系良好,平均加样回收率分别为97.86%(RSD=1.25%)、97.39%(RSD=0.68%)。测定5批次康复新液中尿苷和黄嘌呤的含量范围分别为1.69~1.76μg·mL^(-1)、12.76~13.06μg·mL^(-1),说明各批样品质量较均一。结论:本文建立的方法精密度高、重复性好,操作简便快速,可用于康复新液的质量控制。
Objective： To establish a method to detect xanthine and uridine content in Kangfuxin Ye at the same time. Method： HPLC method was established. The separation was carried out on a Diamonsil C18 column （250 mm×4.6 mm, 5 μm） at 254 nm. The mobile phase was consist of MeOH and H20 （5：95） at the flow rate of 1 mL.min-1, and the column temperature was set at 30℃. Result： The linear ranges of uridine and xanthine were 0.10-3.96 μg. mL-1 （r2=0.9993） and 0.52-20.80μg. mL-1 （r2=0.9991）, respectively. The average recoveries of uridine and xanthine were 97.86% （RSD=1.25%） and 97.39% （RSD=0.68%）, respectively. The method was applied in the determination of 5 batches of Kangfuxin Ye. The content ranges of uridine and xanthine werel.69- 1.76 μg. mL-1 and 12.76- 13.06 μg. mL-1, respectively, which suggested that the quality of each batch was uniform. Conclusion： The method has high precision, good repeatability, simple and rapid operation. It can be used to control the standard of Kangfuxin Ye.
出处
《中药与临床》
2017年第2期34-37,共4页
Pharmacy and Clinics of Chinese Materia Medica
基金
成都中医药大学科技发展基金(030018052)
