双酚A和苯酚对日本沼虾幼虾的急性联合毒性效应

Joint toxicity of bisphenol A and phenol to the juvenile prawn of Macrobrachium nipponense

采用静水生物测试法研究了双酚A和苯酚对日本沼虾(Macrobrachium nipponense)幼虾单一因子的急性毒性效应,在此基础上,运用毒性单位法研究了双酚A和苯酚对日本沼虾幼虾联合毒性效应。试验结果表明,双酚A和苯酚的浓度与日本沼虾幼虾的死亡率之间呈显著正相关,随着染毒时间的延长,延长半数致死浓度逐渐降低,且存在较显著的剂量—效应和时间—效应关系。分别作用24 h和48 h,双酚A对日本沼虾幼虾的LC_(50)分别为25.68和12.03 mg/L,苯酚对日本沼虾幼虾的LC_(50)分别为33.63和27.59 mg/L。双酚A和苯酚对日本沼虾幼虾的安全浓度分别为0.80和5.57 mg/L。双酚A与苯酚联合作用24 h和48 h对日本沼虾幼虾的LC_(50)分别为0.97 TU和0.51 TU,TUi均小于1,两者的联合毒性效应都表现为协同作用。

A static bioassay test was used to study the cute toxicity of BPA（Bisphenol A） and phenol to the juvenile prawn of Macrobrachium nipponens, and based on which, toxic unit （TU） method was used to study the joint toxicity of BPA and phenol to Macrobrachium nipponense.The results showed that there were significant relationships between the death rate of the juvenile prawn and the concentrations of BPA and phenol.The LC50 decreased gradually with the increasing of the exposure time, and there were significant dose-effect and and time-effect.The 24 h LC50 and 48 h LC50 of the juvenile prawn to BPA were 25.68, 12.03 mg/L, respectively, and 33.63, 27.59 mg/L to phenol, respectively.The safe concentrations of the juvenile prawn to BPA and phenol were 0.80 and 5.57 mg/L, respectively.The LC50 of 24 h and 48 h joint toxicity of BPA and phenol to the juvenile prawn were 0.97 and 0.51 TU, respectively.The TUi was less than 1, which meant that the joint action type is synergistic.