毕节市七星关区臭豆腐质量检测

The Quality Testing About of the Stinky Tofu in Bijie City

选取贵州毕节市区毛霉长约1 cm的臭豆腐,利用平板法从臭豆腐菌落中分离、纯化得到优良毛霉菌落。利用正交分析,对毛霉产蛋白酶的液体发酵条件进行研究发现,在培养温度51℃、培养基pH值7.3、培养时间50h条件下,是毛霉产蛋白酶的活性最高,有利于快速水解蛋白质。

The crude protease, produced by Mucor sp. , was extracted from strong - smelling preserved Stinky Tofu, whose length was over than 1 cm in Bijie City. The strong - smelling preserved bean curd were isolated and purified gain excellent mucor colony by platemethod. By orthogonal analyse, on mucor protease produced by liquid fermentation were studied, the Mucor protease maximum activity of conditions is temperature of 51 ℃, pH 7.30 and culture time 50h, facilitates the rapid hydrolysis of protease.