炮天雄和刨附片对小鼠免疫功能的影响

Immunoregulation effect of Pao Tianxiong and Bao Fupian in mice

目的:探讨炮天雄与刨附片对小鼠免疫功能的影响。方法:采用标准物提取法制备炮天雄、刨附片水煎液。取正常小鼠,分组后,给予灌胃炮天雄或刨附片5.0、2.5、1.25 g.kg^(-1),连续30天或15天,应用碳粒廓清实验或脏器指数实验,考察炮天雄和刨附片对正常小鼠的非特异性免疫;此外,用不同剂量的炮天雄、刨附片水煎液灌胃免疫抑制小鼠11天,测定小鼠碳粒廓清吞噬能力、外周血细胞水平及肝、脾、胸腺脏器指数。结果:刨附片5.0 g.kg^(-1)能显著提高正常小鼠碳粒廓清指数K(P<0.05);炮天雄5.0、2.5、1.25 g.kg^(-1)和刨附片5.0、2.5、1.25 g.kg^(-1)对免疫低下小鼠的胸腺指数、脾指数均有升高趋势;刨附片2.5 g.kg^(-1)能显著性升高免疫低下小鼠的肝脏指数(P<0.05)。结论:刨附片和炮天雄对正常小鼠和免疫低下小鼠有一定增强非特异性免疫功能的作用。

Objective： To observe the immunoregulatory effect of Pao tianxiong （PTX） and Bao Fupian （BFP）. Method： The water decoction of PTX and BFP were prepared using standard extracts method. After grouping mice, different groups were given PTX or BFP in the doses of 5.0 ·kg^-1, 2.5 ·kg^-1, 1.25 ·kg^-1 for 30 days or 15days. Carbon clearance test and immune organ index were used to inspect the effect of PTX and BFP on non-specific immunity on normal mice. Moreover, immunosuppression mice were gavaged in different doses of PTX and BFP for 11 days. The immunity indices such as macrophage phagocytosis, peripheral blood cell levels and immune organ indices were tested. Result： 5.0 g-kg! BFP can significantly increase the carbon clearance index K in normal mice （P〈0.05）. PTX in the doses of 5.0, 2.5, 1.25 g·kg^-1 and BFP in the doses of 5.0, 2.5, 1.25 g·kg^-1 had a trend of increasing thymus index and spleen index in inlmunosuppression mice. 2.5 g·kg^-1 BFP significantly increased liver index in immunosuppression mice （P〈0.05）, Conclusion： BFP and PTX have certain effect on strengthening the immune function both in normal mice and immunosuppression mice.