阿里红多糖体外免疫活性的研究

Study on Immune Activity of Fomes Oficinalis Sporophore Polysaccharide in Vitro

目的:探讨阿里红多糖(FoP)对小鼠体外免疫的调节作用。方法:采用体外淋巴细胞增殖实验考察FoP对小鼠脾淋巴细胞增殖的影响;采用磺酰罗丹明B(SRB)法检测不同浓度FoP单独作用及其与有丝分裂原ConA、LPS共同作用对小鼠脾淋巴细胞增殖的影响;采用酶联免疫吸附实验(ELSA)法检测不同浓度FoP体外刺激对小鼠脾淋巴细胞诱生白介素2(IL-2)、白介素13(IL-13)、肿瘤坏死因子α(TNF-α)等细胞因子的影响。结果:Fop在25~100μg/m L浓度范围内能显著促进小鼠脾淋巴细胞增殖,并与Con A、LPS协同促进T、B淋巴细胞增殖,还能诱导脾淋巴细胞分泌IL-2、TNF-α。结论:FoP具有免疫促进作用。

Objective： To explore the immunoregulation offomes oficinalis sporophore polysaccharide （FOP） on the mice in vitro. Methods： Lymphocyte proliferation test was adopted to investigate the effects of FoP on the proliferation of splenic lymphocyte of the mice; and SRB method was used to detect the act of FoP independently in different concentrations and its combined action with the mitogen ConA, LPS on splenic lymphocyte of the mice; ELISA method was adopted to survey the effects of FoP with different concentrations in vitro stimulating splenic lymphocyte inducing the cell factors such as IL-2, IL-13, TNF-a and others. Results： FoP could promote the proliferation of splenic lymphocyte of the mice in the concentrations ranged between 25 and 100 μg/mL, and promote T and B lymphocyte proliferation by working with ConA, LPS, and induce splenic lymphocyte secreting IL-2 and TNF-a. Conclusion： FoP plays a role in immunological enhancement.