基于Luminol的发光体系检测元胡中延胡索乙素

Determination of tetrahydropalmatine in Corydalis yanhusuo by chemiluminescence systems based on Luminol

Luminol-KMnO_4-OH^-、Luminol-NaIO_4和KMnO_4-Luminol-H^+发光体系在碱性或酸性条件下能产生化学发光,加入延胡索乙素后可抑制体系的发光强度,据此建立了流动注射-化学发光检测元胡中延胡索乙素的分析方法,同时开展了方法验证和干扰试验,并对3种体系分别进行了流路及条件优化。在优化条件下,Luminol-KMnO_4-OH^-、Luminol-NaIO_4和KMnO_4-Luminol-H+体系的相对发光强度分别与延胡索乙素浓度在一定范围内呈良好线性关系。试验结果表明,方法的检出限分别为0.029 66μg/m L、0.008 69μg/m L、0.041 38μg/m L,平均回收率≥97.66%;检测样品中延胡索乙素平均得率分别为0.0618%、0.0612%、0.0611%。所构建的3种化学发光体系的线性检测范围宽、检出限低、精密度好,研究结果可用于中药材元胡的品质分析与质量检测。

The three chemiluminescence （CL） systems, including Luminol-KMnO4-OH-, Luminol- NaIO4 and KMnO4-Luminol-H＋ , can produce obvious CL reaction in the alkaline or acidic medium, while the tetrahydropalmatine can inhibit those CL signal of these systems. Based on that, a method was established for detecting the tetrahydropalmatine in Corydalis yanhusuo by chemiluminescence-flow injection analysis （CL- FIA）, carried out the method validation and interference test, and 3 kinds of systems had conducted flow and parameter optimization. Under the optimized test conditions, the concentration of tetrahydropalmatin was show- ing a good linear relationship with the relative luminous intensity in three systems. Above all, detection limit of three systems was 0.029 66μg/mL, 0.008 69 μg/mL and 0.041 38μg/mL respectively, and the average rate of recovery was over 97.66%. The average extraction ratio of tetrahydropalmatine in the detecting samples with those three systems was 0. 0618% , 0. 0612% and 0. 0611% , respectively. Based on those results, it was concluded that the established method was showing the wide linear detection range, low detection limit and high precision, and the results can be used to quality analysis of Corydalis yanhusuo, and detecting tetrahydro- palmatine practically in Corydalis yanhusuo.