耐力训练C57BL/6J小鼠工作肌ATGL的变化及信号分子cAMP和PPAR-γ的影响

The Effect of Signal Molecule cAMP and PPAR-γ on ATGL of Working Muscular in C57BL/6J Mouse in Exercise Training

目的:1)了解耐力训练不同时间点C57BL/6J小鼠ATGL基因和蛋白表达的变化;2)腹腔注射c AMP的激活剂Forskolin、PPAR-γ的抑制剂T0070907、观察耐力训练后ATGL的表达,探究ATGL变化可能的信号分子机制。方法:实验1,C57BL/6J小鼠跑台耐力训练训练4周,使用实时荧光定量PCR和蛋白质印迹法测定腓肠肌和比目鱼肌ATGL基因和蛋白表达的变化;实验2,采用跑台耐力训练小鼠4周,分别每天腹腔注射forskolin、T0070907和DMSO,测定腓肠肌和比目鱼肌ATGL基因和蛋白表达的变化。结果:1)耐力运动引起比目鱼肌ATGL基因和蛋白表达量出现上升(P<0.05),并且在第4周运动组的ATGL基因表达量显著高于对照组(P<0.05);第3、第4周ATGL蛋白表达都显著高于对照组(P<0.05);2)耐力运动时腓肠肌ATGL基因和蛋白表达出现上升(P<0.05),耐力训练第3周和4周ATGL基因和蛋白表达量运动组显著高于对照组(P<0.05);3)4周的耐力运动训练后,forskolin组的ATGL基因和蛋白表达无论是比目鱼肌还是腓肠肌都出现显著高于对照组(P<0.05),T0070907组的腓肠肌中ATGL基因和蛋白表达显著低于对照组(P<0.05);4)在4周的耐力运动训练后,forskolin组的ATGL基因表达腓肠肌显著高于比目鱼肌(P<0.05)。结论:耐力训练会引起C57BL/6J小鼠比目鱼肌和腓肠肌中的ATGL基因和蛋白表达上升,信号分子c AMP和PPAR-γ参与了这一过程。

Objective： 1 ） The purpose of this study was to observe the changes ofATGL gene and protein expression in C57BL/6J mice at different time points in endurance training; 2） To explore the possible molecular mechanism of ATGL changes after intraperitoneal injection of cAMP activa- tor Forskolin, PPAR- inhibitor T0070907. Methods： Experiment 1, Endurance training last for four weeks of training C57BL / 6J mice, the gastrocnemius and soleus muscle of ATGL gene and protein expression was determined by real-time fluorescence quantitative PCR and Western blotting; Ex- periment 2, ATGL gene and protein expression in gastrocnemius and soleus muscle are determined respectively daily intraperitoneal injection of forskolin, T0070907 and DMSO after 4 weeks tread- mill endurance trained mice. Results： 1 ） ATGL gene and protein of the soleus muscle expression increased in endurance exercise （P〈 0.05 ）, and the ATGL mRNA expression in exercise group was significantly higher than the control group in fourth week （P〈 0.05）, the expression of ATGL pro- tein was significantly higher than that of control group in＇the third, and in the fourth weeks （P〈 0.05 ） 2） ATGL mRNA and protein expression in the gastrocnemius muscle increased during endurance exercise （P〈 0.05 ）, ATGL mRNA and protein expression in exercise group was significantly higher than those in the control group in the third week and the fourth week of endurance training （P〈 0.05 ） 3 ） ATGL gene and protein expression of soleus and gastrocnemius muscle in forskolin group for 4 weeks endurance training were significantly higher than those of the control group （P〈 0.05）, AT- GLmRNA and protein of T0070907 group in the gastrocnemius muscle was significantly lower than those of the control group （P〈 0.05 ）; ATGL gene expression in forskolin group was significantly higher than that of gastrocnemius muscle after 4 weeks endurance training （P〈 0.05）. Conclusion： Endurance training can lead to the increasement of ATGL gene and protein expression soleus and gastrocnemius muscle in the C57BL/6J mouse, which the cAMP and PPAR-y, signal molecule are involved in.