姜黄素对周围神经再生和S100表达的研究

Effect of curcumin on peripheral nerve regeneration and S100 expression

目的探讨周围神经横断损伤后,姜黄素对周围神经再生的影响及其机制。方法将BALB/c小鼠(N=200)随机分为正常对照组(0mg/kg/天)、模型对照组(0mg/kg/天)、低剂量组(10mg/kg/天)、中剂量组(20mg/kg/天)、高剂量组(40mg/kg/天),每组40只,后4组建立坐骨神经横断损伤模型,姜黄素胃内给药1周。在姜黄素给药后的第1、2、4、8周,每组均进行电神经生理检测、免疫组织化学染色、Luxol Fast Blue(LFB)染色、实时定量聚合酶链反应(qPCR)、Western Blotting检测。结果电神经生理检测显示,每组动作电位幅度和运动神经传导速度(MNCV)从第1周到第8周呈递增趋势,每周中剂量组和高剂量组的增幅大于低剂量组和模型对照组(P<0.05),高剂量组和低剂量组之间、低剂量组和模型对照组之间电生理特征差异无统计学意义(P>0.05);免疫组织化学染色显示,中剂量组和高剂量组的染色强度明显强于低剂量组和模型对照组;LFB染色显示,中剂量组和高剂量组重生有髓纤维的直径和数量明显多于低剂量组和模型对照组;qPCR和Western Blotting检测L4-6脊髓节段S100的表达水平,结果显示中剂量组和高剂量组S100的表达水平明显强于低剂量组和模型对照组(P<0.05)。结论本研究结果表明,姜黄素可通过上调S100的表达,以浓度依赖性方式,促进横断周围神经的再生。本研究的结果有助于开发利用姜黄素治疗周围神经损伤。

Objective To investigate the effect of curcumin on peripheral nerve regeneration after peripheral nerve transection and its mechanism.Methods BALB/c mice（N=200）were randomly divided into normal control group（0 mg/kg/day）,model control group（0 mg/kg/day）,low dose group（10 mg/kg/day）,middle dose group（20 mg/kg/day）,high dose group（40 mg/kg/day）,and each group of 40.BALB/c mice underwent complete sciatic nerve amputation,and followed by an immediate epineurium anastomosis.Mice were intragastrically administered curcumin at doses of 40（high）,20（moderate）,and 10 mg/kg/d（low）for 1week.Following the 1st,2nd,4th and 8th week after curcumin administration were electroneurophysiological tests,immunohistochemical staining,Luxol Fast Blue（LFB）staining,real-time quantitative polymerase chain reaction（qPCR）and Western Blotting assay each group.Results Electroneurophysiological tests showed that the amplitude of each action potential and motor nerve conduction velocity（MNCV）were increasing from the first week to the eighth week.The increase of the middle dose group and the high dose group was higher than that of the low dose group and the model control group（P〈0.05）.There was no significant difference in electrophysiological characteristics between high dose group and low dose group or low dose group and model control group（P〉0.05）.Immunohistochemical staining showed that the staining intensity of the middle dose group and the high dose group was significantly higher than that of the low dose group and the model control group.Luxol Fast Blue（LFB）staining showed that the diameter of the myelinated fibers in the middle and high dose groups.QPCR and Western Blotting were used to detect the expression of S100 in L4-6 spinal cord segment.The results showed that the expression level of S100 in middle dose group and high dose group was significantly higher than that in low dose group and model control group（P〈0.05）.Conclusion The results of this study show that curcumin can promote the regeneration of peripheral nerves by up-regulating the expression of S100 in a concentration-dependent manner.The results of this study may help to develop the use of curcumin in the treatment of peripheral nerve injury.