产果胶酶黑曲霉的UV诱变及基于葡萄皮渣的固态发酵

UV mutagenesis of Aspergillus niger for increasing pectinase production and its solid-state fermentation using grape skin residue

对产果胶酶的黑曲霉进行紫外诱变,使用透明圈法并结合液态发酵法进行筛选,获得果胶酶产量提高的黑曲霉菌株,并利用葡萄皮渣进行固态发酵生产果胶酶。结果表明,H40741、H40493、H40982、H41133、H41134、H41034、H40493和H22148等8株黑曲霉中,H40741产果胶酶最高,对其进行0~360 s紫外诱变,获得高产果胶酶突变菌株H40741-20,其在果胶液态发酵培养基中可获得酶活力为462.84 U/m L的果胶酶,是出发菌株的3倍。通过优化固体发酵培养基中的葡萄皮渣、麦麸和水的含量,获得较佳培养基配方为:葡萄皮渣50 g,麸皮50 g,水100 m L,硫酸铵2 g,硫酸镁0.07 g,硫酸钾0.1 g。H40741-20利用此固态培养基发酵,可获得酶活力为421.06 U/m L果胶酶,是出发菌株H40741的2.7倍。

For increasing pectinase productiont, UV light was used to produce AspergiUus niger mutagenesis, and the grape skin residue was used to produce peetinase by Aspergillus niger. The results showed that, in the eight Aspergillus niger strains H40741, H40493, H40982, H41133, H41134, H41034, H40493 and H22148, the H40741 has the highest pectinase activity. Ultraviolet light on H40741 spores 0-360 s, and we obtained a high yield pectinase mutant strain H40741-20.The pectinase activity was 462.84 U/mL in pectin liquid fermentation medium, which was 3 times of the original strain. By optimizing the contents of grape skin residue,wheat bran and water,we obtained a better medium formula was grape skin residue 50 g,wheat bran 50 g, water 100 mL, ammonium sulfate 2 g, magnesium sulfate 0.07 g, potassium sulfate.The pectinase enzyme activity was 421.06 U/mL,which was 2.7 times higher than that of the original strain H40741 by using this optimized solid medium.