ZnO溶胶的制备及其抑菌性和光催化性能研究

Preparation of ZnO sol and its performance for bacteriostasis and photocatalytic degradation of organics

以二水合醋酸锌和KOH为原料,聚乙烯吡咯烷酮(PVP)为表面修饰剂,制备了稳定透明的ZnO溶胶。利用X射线衍射仪、扫描电子显微镜、紫外可见分光光度计、荧光光谱仪和高效液相色谱仪对其进行了表征和测试。考察了二水合醋酸锌、KOH和PVP的用量对ZnO粒径及溶胶稳定性的影响;以金黄色葡萄球菌、大肠杆菌、绿脓杆菌、洋葱假单胞菌、白色念珠菌和黑曲霉为测试对象,采用琼脂稀释法测定ZnO溶胶的最小抑菌浓度(MIC);并对亚甲基蓝、乐果和乙酰甲胺磷溶液进行光催化性能研究。实验结果表明:在添加10 mmol二水合醋酸锌、15 mmol KOH和1.6 g PVP的较佳条件下合成的ZnO溶胶最为稳定,ZnO粒径分布均匀;ZnO溶胶有效地抑制了菌种的生长,其对金黄色葡萄球菌和洋葱假单胞菌的抑菌效果最好;同时高效地降解了亚甲基蓝、乐果和乙酰甲胺磷溶液,表现出较好的光催化活性。

Stabilized and transparent ZnO sol was prepared with zinc acetate dihydrate （ZAD）and potassium hydroxide （KOH）as starting materials and polyvinyl pyrrolidone （PVP）as surface modifying agent. The product was characterized and tested by X-ray diffraction,scanning electron microscopy,UV-visible absorption spectroscopy,fluorescence spectroscopy and high performance liquid chromatography. The influences of ZAD,KOH and PVP dosage on ZnO particle size and sol stability were examined. The minimum inhibitory concentration （MIC）value of ZnO sol were determined by agar dilution method with Staphylococcus aureus, Escherichia coli,Agrobacterium tumefaciens,Pseudomonas cepacia,Candida albicans and Aspergillus niger as testing objects. The photocatalytic activity of ZnO sol on degradation of methylene blue （MB）,dimethoate and acephate solution was carried out. Results show that the ZnO sol product prepared with the stoichiometric composition of 10 mmol of ZAD,15 mmol of KOH and 1. 6 g of PVP displays stable and uniformly distributed ZnO nanoparticle size. The growth of bacteria is inhibited effectively by the ZnO sol product,which has the best antibacterial effect on Staphylococcus aureus and Pseudomonas cepacia. Meanwhile,the ZnO sol product displays good photocatalytic activity on degradation of MB,dimethoate and acephate solution.