顶空固相微萃取结合GC/MS分析玫瑰香型滇红茶香气成分

Analysis of volatile aroma components in rose aromatic Yunnan black tea using headspace solid-phase microextraction coupled with GC-MS

采用顶空固相微萃取(HS-SPME)结合气相色谱-质谱联用技术(GC-MS)对玫瑰香型滇红茶样品中的挥发性香气成分进行分析,同时与普通滇红茶的挥发性成分进行比较。实验首先对HSSPME参数进行优化,确定了HS-SPME最优参数为:65μm PDMS/DVB萃取头,萃取温度60℃,萃取时间60 min。从玫瑰香型滇红茶和普通滇红茶中分别检测出62种和58种香气成分,各占其总香气成分的93.23%和93.85%,主要为醇类、碳氢类、酯类、酮类、醛类、酸类和含氮类化合物。2种茶叶中具有玫瑰香或类似玫瑰香的化合物共6种,分别为芳樟醇、苯乙醇、香叶醇、β-大马烯酮、香叶基丙酮、(E)-橙花叔醇,在玫瑰香型滇红茶和普通滇红茶中的总相对含量分别为36.29%和15.51%,玫瑰香型滇红茶6种化合物的总相对含量约为普通滇红茶的2.2倍。该研究结果为玫瑰香型滇红茶香气的进一步研究提供了一定的理论依据。

The aroma compounds of rose aromatic Yunnan black tea were analyzed by head-phase microextraction （HS-SPME） coupled to gas chromatography-mass spectrometry （GC-MS）, and compared with the common Yunnan black tea. The parameters of HS-SPME were initially evaluated. The optimal extraction parameters were achieved under the following conditions： 65 pm PDMS/DVB fiber, 60 ℃ extraction temperature, 60 min extraction time. Under optimal conditions, 62 and 58 kinds of compounds were identified in rose aromatic and common Yunnan black tea, accounting for 93.23% and 93.85%, respectively. The major constituents were alcohols, hydrocarbons, esters, ketones, aldehydes, acids, and nitrogen compounds. Six compounds with rose aromatic or similar with rose aromatic were found in both Yunnan black tea samples, there were linalool, phenylethyl alcohol, geraniol, 13-damascenone, geranyl acetone, （E）-nerolidol. The total relative content of 6 compounds in rose aromatic and common Yunnan blacktea were 36.29% and 15.51%, the relative content of rose aromatic Yunnan black tea is 2.2 times as big as that of common Yunnan black tea. These results could provide a theoretical basis for the further study of aroma of rose aromatic Yunnan black tea.