鼠伤寒沙门氏菌/哺乳动物微粒体酶试验检测生命壹号口服液安全性

Safety test of life one oral liquid by Salmonella typhimurium/mammalian microsomal enzyme test

目的采用鼠伤寒沙门氏菌/哺乳动物微粒体酶试验(Ames)对生命壹号口服液进行安全性毒理学试验。方法实验选用鼠伤寒沙门菌TA97、TA98、TA100和TA102菌株,将生命壹号口服液配制成浓度为50000、10000、2000、400、80μg/mL的溶液,作为5个剂量组,同时设未处理对照组、溶剂对照组(二甲基亚砜)和阳性对照组(2-乙酰氨基芴、4-硝基喹啉-N-氧化物),观察各菌株的回变菌落数。结果生命壹号口服液Ames试验各剂量组的回变菌落数均未超过未处理对照组自发回变菌落数的2倍,Ames实验结果为阴性。结论在本实验条件和剂量范围内,未观察到生命壹号口服液对鼠伤寒沙门氏菌TA97、TA98、TA100、TA102的诱变作用。

Objective To investigate the safety toxicology test of life one oral liquid by Salmonella typhimurium/mammalian microsomal enzyme test（Ames）. Methods Salmonella typhimurium strains of TA97, TA98, TA100 and TA102 were selected, and oral solution was prepared into concentrations of 50000, 1000, 2000, 400, 80 μg/mL as for the 5 dose groups. The untreated control group, solvent control group and positive control groups were set to observe the number of colonies of each strain. Results In the Ames test, the number of converted colonies in each dose group was no more than 2 times of that of the untreated control group. The results of Ames test were negative. Conclusion In the experimental conditions and dose range, the effect of life one oral liquid on TA97, TA98, TA100 and TA102 of Salmonella typhimurium is not observed.