超高液相色谱串联质谱法检测土豆及土豆制品中α-茄碱

Determination of α-solanine in potato and its products by ultra-high performance liquid chromatography tandem mass spectrometry

目的建立超高效液相色谱串联质谱法检测土豆及其制品中α-茄碱的分析方法。方法通过优化提取液,土豆样品经1%甲酸-甲醇(1:1,V:V)超声提取,采用BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm)分离,梯度洗脱,采用超高效液相色谱串联质谱法,在多反应监测模式进行定性和定量分析,而土豆淀粉用MCX固相萃取柱进行净化,液相色谱串联质谱法测定。结果土豆样品中回收率为75%~115%,相对标准偏差为5.0%~8.6%,土豆淀粉的回收率范围为67%~100%,相对标准偏差为3.4%~11.3%,方法的检出限和定量限分别为0.5 mg/kg和1.0 mg/kg。结论该方法适合于土豆及其制品中α-茄碱的分析。

Objective To establish a method for the determination of α-solanine in potato and its products by ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. Methods Potato samples were extracted by the mixture of 1% formic acid and methanol（1：1, V：V）, and separated by BEH C_（18） column（2.1 mm×50 mm, 1.7 μm） with gradient elution. Multiple reaction monitoring model was used for qualitative and quantitative analysis. The potato starch was extracted and purified by MCX solid phase extraction and determined by UPLC-MS/MS. Results The recoveries of potato samples were 75%~115% with the relative standard deviation in the range of 5.0%~8.6%. The recoveries of potato starch were 67%~100% with the relative standard deviation in the range of 3.4%~11.3%. The limit of detection and the limit of quantification were 0.5 mg/kg and 1.0 mg/kg, respectively. Conclusion The method is suitable for determination of α-solanine in potato and its potato products.