枸杞多糖对生精障碍模型小鼠生精能力作用的研究

Function of spermatogenic promotion of lycium barbarum polysaccharide on mice with spermatogenesis impairment

目的研究枸杞多糖(LBP)对生精功能和生精干细胞的影响。方法 122只雄性昆明小鼠中随机选取92只,通过35 mg/kg白消安造模,获得生精障碍动物模型,另外30只小鼠为正常对照组。40 d后对造模成功的小鼠随机分为自然恢复组(n=23)和LBP低剂量组(n=16,75 mg/kg)、中剂量组(n=19,150 mg/kg)、高剂量组(n=16,300 mg/kg),连续灌胃给药70 d(小鼠2个生精周期)后进行观察,并与正常对照组进行比较,观察其生理状态和生殖功能变化。结果 LBP在造模动物的外观体征、机能和行为的表现、动物正常发育状态(体质量)、生殖器官组织重量(睾丸、附睾和储精囊、前列腺-会阴复合体)、附睾精子质量、睾丸组织精子生成量、睾丸组织形态学和c-kit表达量、精曲小管直径和空泡化程度、生精上皮厚度和细胞层数、c-kit阳性细胞数、H-score评分、平均吸光度(IA)等系列指标均显示明显高于自然恢复组(P<0.05,P<0.01),并具有剂量效应关系,但仍未达到正常对照动物的水平。结论 LBP具有明显促进白消安生精障碍模型小鼠的生精能力的作用,可以显著增加生精干细胞、附睾精子质量和睾丸精子生成量。

Objective To study the effect of lycium barbarum polysaccharide （LBP） on the function of spermatogenesis and spermatogonial stem cells. Methods Totally 92 of 120 male Kunming mice were randomly selected and treated with 35 mg/kg busulfan to build the animal model with spermatogenesis impairment. And 14 mice were selected as solvent control, 16 selected as normal control. After 40 d, the model animals were randomly divided into spontaneous recovery group （n=23）, LBP low-dose group （n=16, 75 mg/kg）, LBP middle-dose group （n=19, 150 mg/kg）, LBP high-dose group （n=16, 300 mg/kg）. After treatment for 70 d （two spermatogenic cycles）, the changes of physiological status and reproductive function were observed. Results In terms of the appearance, behaviors, body weight, reproductive organs, sperm count in epididymis, sperm count in testis, histology and morphology of testis and expression of c-kit, three kinds dosages of LBP group were better than the spontaneous recovery group with dose-effect relationship, but still not achieve the level of normal control. Conclusion LBP can promote the spermatogenic function on the busulfan-treated infertile mouse, and increase the spermatogonial stem cells, quality of sperm in epididymis and sperm count in testis.