沙眼衣原体感染不育男性的精液质量与IL-6、IL-8水平的相关性分析

Correlation analysis between semen quality and the levels of seminal IL-6 and IL-8 in Chlamydia trachomatis infection-caused male infertility

目的探讨沙眼衣原体感染不育男性的精液质量与IL-6、IL-8水平的关系。方法选择2015年2月至2016年6月我院收治的不育男性250例,收集患者的血液、首段尿(FVU)和精液标本,分别检测血清特异性Ig A、Ig M、Ig G抗体、FVU和精液沙眼衣原体DNA、精液IL-6、IL-8浓度,并进行精液常规分析。分析沙眼衣原体感染患者的IL-6、IL-8水平变化,及与精液常规参数等指标的相关性。结果 (1)尿液、血清衣原体阳性患者IL-6和IL-8水平与正常生育男性比较均明显升高,差异有统计学意义(P<0.05)。(2)IL-6和IL-8水平与白细胞浓度均具有相关性,且IL-8水平与精液体积和患者年龄相关。结论 FVU沙眼衣原体阳性的男性精液体积明显低于阴性者,IL-6和IL-8水平与白细胞浓度呈正相关。IL-8水平与精液体积呈负相关而与男性年龄呈正相关。

Objective To study the relationship between semen quality and the level of seminal IL-6 and IL-8 in C. trachomatis infection-caused male infertility. Methods Total of 250 infertile men were recruited in the study, and their blood, first void urine （FVU） and semen samples were collected. Analysis for specific IgA, IgM and IgG antibodies to C. trachomatis in serum, the presence of C. trachomatis in FVU and semen sample and semen analysis were carried out. IL- 6, IL-8 levels and semen parameters were comparatively analyzed in C. trachomatis infection-caused infertile male. Results （1） Chlamydia trachomatis positive male seminal plasma IL-6 and IL-8 levels increased, but no significant difference, and with the diagnosis method. （2） IL-6 and IL-8 levels were associated with leukocyte concentrations, and IL-8 levels were associated with semen volume and patient age. Conclusion This study showed that semen volume of C. trachomatis infection- positive infertile male was lower than that of the men without infection. Relationship among IL-6, IL-8 level, and leukocyte were observed, and IL-8 level was negatively correlated with semen volume, while positively correlated with age.