真武汤对心力衰竭大鼠心肌中小G蛋白的影响

Influence of Zhenwu Decoction on small G protein in cardiac muscle of Heart Failure

目的通过超声检测,使真武汤在治疗冠心病后心衰的效果更直观,探讨其机制并明确的指导临床用药。方法选取健康雄性wistar大鼠60只,随机选取50只按大鼠进行心梗后心力衰竭造模手术。把造模大鼠随机平均分成5组,分别为模型组,真武汤组(高、中、低剂量),卡托普利组,其余10只为空白对照组。将这些大鼠分别灌胃15天进行超声检测,计算出左室短轴缩短率(LVFS)与左室射血分数(LVEF)。取样本用westen blot技术检测心肌中小G蛋白相关变化。结果高剂量真武汤组LVFS与LVEF值较模型组相比改善,P<0.01有统计学意义;高剂量组灌胃的大鼠小G代表灰度值表达趋势降低,P<0.01。说明真武汤可以减少小G的活化,真武汤可以缓解心衰患者临床症状,改善心功能。

Objective By echocardiograph,the effect of Zhenwu Decoction is more intuitive in treating heart failure,Clear guidance for clinica. Methods Selecting 60 male wistar rats,We randomly selected 50 male wistar rats building operation,being heart failure after heart attack. The rats were randomly assigned to five groups,For the model group,the Zhenwu Decoction group（ Zhenwu Decoction high-dose,moderate-dose,low-dose groups）,the captopril group,the other 10 rats were just blank control groups. This group rats were given by gavage once a day for 15 d. The blank group was filled with saline. By echocardiograph,to calculatet the LVFS and LVEF. The sample was used to detect changes small G protein in the heart muscle of the westen blot technique. Results The high-dose group＇s LVFS and LVEF were significantly better than the model group,P 0. 01,has statistical significance. The high-dose group＇s rat＇s small G protein show significantly lower trends,P 0. 01. Zhenwu Decoction is not onlyeffective in alleviating clinical symptoms,but also improving the function of the heart.