锁阳80%乙醇提取物对去卵巢大鼠骨质疏松防治作用研究

The effect of Cynomorium songaricum Rupr. on osteoporosis in ovariectomized rats

目的:考察锁阳80%乙醇提取物对去卵巢大鼠骨质疏松的防治作用,通过对骨形成和骨吸收指标的测定探讨锁阳抗骨疏松可能的作用机制。方法:3月龄SD雌性大鼠随机分为5组:分别为去卵巢模型组(0.5%CMC-Na,10 ml/kg)和假手术组(0.5%CMC-Na,10 ml/kg),戊酸雌二醇组(1 mg/kg),锁阳80%乙醇提取物组(100 mg/kg、300 mg/kg);除假手术组大鼠剖开背部后直接缝合,其它各组大鼠均去除双侧卵巢;术后3天开始灌胃给药,连续12周后取各组大鼠右侧股骨测定总骨密度,显微CT分析骨小梁相关参数;取大鼠血清和尿液,测定钙、磷、等骨矿含量;ELISA法测定血清中骨形成指标碱性磷酸酶(ALP)和骨钙素(BGP)含量,骨吸收指标酒石酸碱性磷酸酶(TRAP)、组织蛋白酶(Cathepsin K)、脱氧嘧啶啉(DPD)含量及抗氧化活性指标。结果:锁阳80%乙醇提取物(100 mg/kg、300 mg/kg)可显著提高去卵巢大鼠骨密度,改善骨质疏松大鼠骨小梁形态结构,明显增加血清中钙和磷的含量;能够显著抑制去卵巢大鼠血清中TRAP和组织蛋白酶K的活性,降低DPD含量,提高ALP酶的活力;同时可显著降低MDA含量。结论:锁阳80%乙醇提取物对去卵巢致大鼠骨质疏松有明显的预防作用,其作用机制可能与加快骨形成、抑制骨吸收及氧化应激有关。

Objective： To investigate the anti-osteoporotic effect of 80% ethanol extracts of Cynomorium songaricum Rupr. in ovariectomized （OVX） rat model, and both bone formation and bone resorption index were used to estimate the underlying mechanisms of the action. Methods： A total of 50 female Spragne-Dawley （SD） rats aged 3 months were randomly divided into five groups： the rats in the Sham control group underwent bilateral laparotomy without removing the ovaries, and the remainders underwent bilateral ovariectomy and equally randomized into model group, 1 mg/kg body weight of estradiol valerate as positive control group, 100 and 300 mg/kg body weight of extracts of Cynomorium songaricum Rupr. as low and high dosage groups, respectively. On the last day of 12 weeks of continues administration, the total bone mineral density （BMD） as well as morphometric parameters were determined by a micro-CT scanner. The levels of serum Ca, P and the activities of alkaline phosphatase （ALP） were measured by automatic analyzer, and deoxypyridinoline （DPD） , tartrate-resistant acid phosphatase （TRAP） , cathepsin K were determined by ELISA. Results： 300 mg/kg and 100 mg/kg dosage groups of the 80% ethanol extracts of Cynomorium songaricum Rupr. significantly enhanced total bone mineral density, improved bone trabecular microarchitecture, increased serum Ca and P content, decreased the activity of bone resorption markers including TRAP, DPD and cathepsin K in OVX rats, as compared with the Sham group. Conclusion：Our data implied that Cynomorium songaricum Rupr. possessed antiosteoporotic effect, and this effect was exerted by the suppressing bone resorption and increasing bone formation, which suggested Cynomorium songaricum Rupr. might be a promising agent for the prevention and treatment of osteoporosis disease.