白豆蔻对阿霉素肾病模型大鼠肾脏组织TGF-β1、PAI-1表达的影响

Effect of Amomumkravanh Pierre ex Cagnep to expression of TGF-β1、PAI-1 on Adriamycin in duced renal injury Rats

目的:研究白豆蔻对阿霉素肾病大鼠的肾脏保护作用及其机制。方法:雄性Wistar大鼠60只,随机分为正常对照组、阿霉素肾病模型组、白豆蔻1.5 g/kg剂量组、白豆蔻3.0 g/kg剂量组、白豆蔻4.5 g/kg剂量组、尿毒清颗粒组。采用尾静脉一次性注射阿霉素(6.5mg/kg)建立阿霉素肾病模型,建立模型后第4d开始灌胃给药,连续给药3W。给药3W后代谢笼留取尿液,麻醉大鼠腹主动脉采血后处死动物并取肾脏组织,通过尿液分析仪检测各组大鼠尿液尿蛋白(PRO)、潜血(BLO)、胆红素(BIL)等指标;ELASE法检测血清Cys-C、尿液β2-MG的含量;免疫组化法检测肾脏组织TGF-β1、PAI-1的表达。结果:与模型组比较,各给药组均有不同程度的降低PRO、BLO、BIL含量,其中白豆蔻3.0 g/kg剂量组的作用优于其它给药组。ELASE法检测结果表明与模型组比较,白豆蔻三个剂量组均有降低Cys-C和β2-MG含量,其中白豆蔻3.0 g/kg剂量组和4.5 g/kg剂量组对Cys-C的作用优于低剂量组,白豆蔻3.0 g/kg剂量组对β2-MG的作用优于其它两个组;免疫组化实验结果表明与模型组比较肾组织TGF-β1、PAI-1的表达白豆蔻4.5 g/kg剂量组比较模型组明显减少,其中白豆蔻的各给药组对TGF-β1的表达没有明显区别。结论:白豆蔻能减轻阿霉素肾病大鼠的肾脏损伤,其作用机制可能与TGF-β1、PAI-1的表达有关。

Objective： To study mechanism and renal protective effects of Amomumkravanh Pierre ex Cagnep （AKP） against Adriamycin induced renal injury in Rats. Methods： Healthy 60 male Wistar rats, were randomly divided into normal group, model group, AKP1.5g/kg dose group, AKP 3.0g/kg dose group, AKP 4.5g/kg dose group, Niaoduqing granule drugs group. Induced renal injury using one time intravenous injection adriamyein （6.5mg o kg-1 ）. establishment of the model After 4d ,administration for 3 consecutive weeks, after 3 weeks collect urine in metabolic cages. Anesthesia abdominal aorta of rats were sacrificed and kidney tissues were collected. Detected urine protein （PRO） , occult blood （BLO） , bilirubin （BIL） of each groups by urine analyzer; Detected the content of Cys-C in serum andβ2-MG in urine by ELASE; Observed pathological changes of renal tissue on light microscopy. Detected expression of PAI-1 （plasminogen activator enzyme） and LN （laminin） in renal tissues by immunohistochemistry. Results： compared with normal group ,the content of PRO,BLO,BIL in model group were increased significantly ; Compared with model group, content of PRO, BLO, BIL in each group increased in different level, the effect of AKP 3.0g/kg dose group is better than other groups. The result of ELASA detection showed that compared with normal group, content of Cys-C in rats serum andβ2-MG in urine were increased significantly; Compared with model group, AKP three dose groups were decreased content of Cys-C andβ2-MG,AKP 3.0g/kg dose group and 4.5g/kg dose group is better than 1.5g/kg does group,the effect of AKP 3.0g/kg close group onβ2-MG is better than other two does groups; HE staining showed that the Adriamycin induced renal damage group and Niaoduqing granule drugs group were havent significantly difference, disease was decreasing when increased dose of AKP three drug group ; The immunohistochemical results indicated that compared with model group,expression of TGF-β1 ,PAI-1 in renal tissues of AKP4.5g/kg dose group was decreased significantly,AKP each does group on expression of TGF-[31 was not significantly different. Conclusion： Amomumkravanh Pierre ex Cagnep （AKP） can decreased Adrianlycin induced renal injury, has a protective effect on kidney.