普通烟草HD-Zip Ⅲ家族全基因组鉴定和表达分析

Genome-Wide Identification and Expression Analysis of the HD-Zip Ⅲ Gene Family in Nicotiana tobacum

植物HD-Zip Ⅲ家族基因在维持分生组织生长、调节避荫反应、决定新生叶背腹性方面起着重要作用。我们通过生物信息学分析对普通烟草(Nicotiana tobacum)TN90基因组序列中的HD-Zip Ⅲ进行广泛预测,并分析了其理化性质、基因结构、进化关系、亚细胞定位、表达谱等信息。本研究在栽培烟草TN90中发现了19个HD-Zip Ⅲ基因的候选序列,通过系统发生分析,可将栽培烟草HD-Zip Ⅲ基因家族成员分为两个亚家族。所有基因参与烟草叶、茎、根、花等各个器官的不同发育时期的表达。这些研究为烟草HD-Zip Ⅲ基因功能的深入分析提供了理论依据。

The HD-Zip Ⅲ gene in plants plays an important role in maintaining the growth of the meristem, regulating the avoidance response, and the decision of the ventral aspect of the newborn. Based on bioinformation analysis, the HD-ZipⅢ genes of cultivated tobacco （Nicotiana tobacum） genome TN90 were screened and selected. The physicochemical properties, gene structure, the evolutionary relationship, subcellular localization and expression pattern were analyzed. A total of 19 HD-Zip Ⅲ genes were identified, the phylogenetic analysis divided the HD-Zip Ⅲ into 2 subfamilies, and all the family members were expressed in at least one tissue in different stages. The research provided the theory foundation for deep analysis of NtHD-Zip Ⅲ gene function.