BR和PEG处理生菜种子对其高温萌发的影响

Effects of BR and PEG Treatment on Germination of Lettuce Seed under High Temperature

为解决华北地区夏季生菜高温生产问题,本研究通过不同浓度的芸苔素内酯和聚乙二醇处理生菜种子,研究对其在高温下的萌发效应。研究发现,经过0.05 mg/kg、0.10 mg/kg和0.15mg/kg浓度的BR浸种处理下,生菜种子30℃、32℃下萌发率降低,BR对生菜种子高温萌发有严重抑制作用。而经过PEG处理的生菜种子在高温下表现优良。跟对照组相比,20℃下,差异不显著;30℃下,15%PEG对生菜种子萌发有抑制作用。其他浓度均促进生菜萌发的作用,其中10%PEG处理组差异最显著,整齐度和发芽率最好;32℃下,除10%PEG差异不显著外,其他所有浓度均促进生菜萌发,其中5%PEG处理组种子活力最高。可见BR不适合作为生菜种子高温下的种子引发剂,而适宜浓度的PEG在对提高生菜种子发芽率和整齐度方面具有显著效果。

In order to solve the problem of summer lettuce production under high temperature in north China, we studied the effects ofbrassinolide （BR） and polyethylene glycol （PEG） with different concentrations on lettuce seeds germination under high temperature. The study found that after soaking treatment with BR 0.05 mg/kg, 0.10 mg/kg, 0.15 mg/kg concentration, lettuce seeds germination rate reduced at 30℃ and 32℃, which indicated that BR had a serious inhibitory effect on lettuce seed germination under high temperature. However, the lettuce seeds treated by PEG showed excellent performance under high temperature. Compared with the control group, the difference was not significant under 20℃ ; Under 30℃, 15% PEG inhibited the germination of lettuce seeds. The other concentrations all promoted the germination of lettuce. Among them, 10% PEG treatment group had the most significant difference, and the uniformity and germination rate of this group were the best; Under 32℃, 10% PEG treatment group had no obvious difference, but other concentrations all promoted the germination of lettuce, with the highest seed vigor in 5% PEG treatment group. In a word, BR was not suitable as a seed initiator for lettuce seeds under high temperature, but PEG with proper concentration had a significant effect on the improvement of lettuce seeds germination percentage and uniformity.