GSTA1基因C-69T多态性与糖尿病视网膜病变的相关性研究

Association Analysis of the Polymorphism of GSTA1 Gene C-69T and Diabetic Retinopathy

糖尿病视网膜病变是糖尿病最常见的微血管病变之一,是工作年龄成人致盲的主要原因。氧化应激是活性氧生成和清除不平衡的结果,在糖尿病视网膜病变的发生中起重要作用。GSTA1是重要的氧化应激相关的谷胱甘肽硫转移酶之一,目前暂没有其多态性与糖尿病视网膜病变发病之间的研究。本实验以546例2型糖尿病样本为研究对象,根据直接眼底镜的结果把病人分成糖尿病视网膜病变组和有糖尿病但没有视网膜病变组。采用构象差异凝胶电泳方法检测GSTA1基因C-69T多态性。GSTA1基因C-69T多态性的CC、CT和TT种基因型在DR组的频率分别为79.8%、17.7%和2.5%,在NDR组的频率分别为77.8%、20.6%和1.6%,两组比较差异无统计学意义(x^2=1.254,p=0.561)。本实验结果表明,GSTA1基因C-69T多态性与中国江西汉族糖尿病视网膜病变的发病没有关联。

Diabetic retinopathy （DR） is one of the most common microvascular complications of diabetes mellitus （DM） and the leading cause of acquired blindness in adults of working age. Oxidative stress is the result of disequilibrium between production and scavenging of reactive oxygen spices （ROS）. It plays an important role in the development of diabetic retinopathy. GSTA1 is one enzyme of glutathione S-transferases, and there are no studys between its polymorphisms and diabetic retinopathy. Based on the results of direct funduscopy detection, 546 cases of 2 diabetes mellitus patients were divided into 2 groups： diabetic retinopathy （DR） group and non-diabetic retinopathy （NDR） group. GSTA 1 C-69T polymorphism were genotyped by PCR conformation difference gel electrophoresis （PCR-CDGE）. The frequencies ofgenotype CC, CT and TT at GSTAI C-69T polymorphism in DR group and NDR group were 79.8%, 17.7%, 2.5% and 77.8%, 20.6%, 1.6%, respectively. There was no significant difference in genotype between DR group and NDR group （χ2=1.254, p=0.561）. The result of the research showed that GSTA1 C-69T polymorphism might be not associated with diabetic retinopathy in Jiangxi Han nationality patients.