急性呼吸道腺病毒感染患儿血清细胞因子的动态变化及临床意义

Dynamic changes and clinical significance of serum cytokines in children with acute respiratory adenovirus infection

目的探讨急性呼吸道腺病毒感染患儿血清细胞因子:肿瘤坏死因子(TNF)、干扰素γ(IFN-γ)、白细胞介素17A(IL-17A)水平的动态变化及其临床意义。方法选取2015年8月-2016年8月内江市中医医院收治的急性呼吸道腺病毒感染患儿17例为观察组,另选17例健康的患儿为对照组,流式液相蛋白定量技术检测患儿血清中TNF、IFN-γ及IL-17A水平变化,分析其临床意义。结果观察组急性期血清中TNF水平显著高于对照组,差异有统计学意义(P<0.05),恢复期TNF水平显著低于急性期,差异有统计学意义(P<0.05),与对照组变化差异无统计学意义(P>0.05);观察组急性期血清中IFN-γ水平显著低于对照组,差异有统计学意义(P<0.05),恢复期IFN-γ水平显著高于急性期,差异有统计学意义(P<0.05),与对照组变化差异无统计学意义(P>0.05);与对照组相比,观察组急性期、恢复期血清中IL-17A水平显著降低,差异有统计学意义(P<0.05),恢复期IL-17A水平显著高于急性期,差异有统计学意义(P<0.05)。结论儿童急性呼吸道腺病毒感染会引起血清细胞因子TNF、IFN-γ、IL-17A水平的动态变化,可能与病情的发生发展有关。

Objective To explore the dynamic changes and clinical significance of serum cytokines [tumor necrosis factor（ TNF）,interferon-γ（ IFN-γ）,interleukin-17A（ IL-17A） ] levels in children with acute respiratory adenovirus infection. Methods A total of17 children with acute respiratory adenovirus infection admitted to the hospital from August 2015 to August 2016 were selected as observation group,and 17 healthy children were selected as control group. The changes of TNF,IFN-γ,and IL-17 A levels in serum were detected by flow cytometry,and the clinical significance was analyzed. Results TNF level in the acute phase of patients in observation group was statistically significantly higher than that in control group（ P〈0. 05）. TNF level in the recovery phase of patients in observation group was statistically significantly lower than that in the acute phase（ P 0. 05）,and there was no statistically significant difference between observation group and control group（ P〈0. 05）. Serum IFN-γ level in the acute phase of patients in observation group was statistically significantly lower than that in control group,and the difference was statistically significant（ P〈0. 05）. IFN-γ level in the recovery phase was statistically significantly higher than that in the acute phase in observation group,the difference was statistically significant（ P〈0. 05）,and there was no statistically significant difference between observation group and control group（ P〈0. 05）. Compared with control group,the levels of serum IL-17 A in the acute phase and recovery stage of patients in observation group decreased significantly（ P〈0. 05）. IL-17 A level in the recovery phase was statistically significantly higher than that in the acute phase,and the difference was statistically significant（ P 0. 05）. Conclusion Acute respiratory adenovirus infection in children may cause the dynamic changes of serum TNF,IFN-γ,and IL-17 A levels,which may be related to occurrence and development of the disease.