中性粒细胞胞外诱捕网对单核细胞分泌炎症介质的影响

The Effect of Neutrophil Extracellular Traps on the Human Monocytes Proinflammatory Cytokines Secretion

目的:探讨中性粒细胞胞外诱捕网(NETs)对单核细胞分泌炎症介质的影响。方法:采用健康人中性粒细胞体外诱导NETs形成,用该NETs刺激单核细胞,LEGENDplex人炎症因子试剂盒检测培养上清多种炎症介质的变化。结果:单核细胞与NETs共培养12 h后,NETs组与对照组相比,IL-1β诱生量分别为(97.60±60.11)pg/ml、<1.17 pg/ml,IFN-α诱生量分别为(208.90±77.38)pg/ml、<1.17 pg/ml,TNF-α诱生量分别为(218.88±86.27)pg/ml、<0.17 pg/ml,MCP-1诱生量分别为(1 063.25±740.01)pg/ml、(157.16±3.86)pg/ml,IL-6诱生量分别为(67.76±15.51)pg/ml、(2.76±0.47)pg/ml,差异均具有统计学意义(P<0.05),IL-10、IL-12表达水平差异无统计学意义。结论:NETs能显著促进单核细胞分泌IL-1β、IFN-α、TNF-α、MCP-1、IL-6。

Objective：Oevaluate the effect of neutrophil extracellular traps （NETs） on the human monocytes proinflammationtory cytokines release. Methods：Neutrophils isolated from healthy human peripheral blood were stimulated with phorbol myristate acetate （PMA）to induce NETs production. After stimulated with NETs, the concentrations of interleukin - 1β （IL - 1β）, interferon - α （ IFN -α）, tumor necrosis factor - α（ TNF -α）, monocyte chemoattractant protein - 1 （ MCP - 1 ）, interleukin - 6 （ IL - 6 ）, interleukin- 10 （IL- 10） and interleukin- 12 （IL- 12） in supernatant were analyzed using LEGENDplex reagents. Results：After twelve hours＇ culture, the proinflammatory cytokines＇ secretion of monocytes stimulated by NETs was significantly higher than that non - stimulated by NETs, including IL - 113 [ （97.60 ± 60.11 ） pg/ml vs 〈 1.17 pg/ml ], IFN - α[ （ 208.90± 77.38 ） pg/ml vs 〈1.17pg/ml], WNF-α[（218.88±86.27）pg/mlvs 〈0.17pg/ml], MCP-1[（1 063.25±740.01）pg/mlvs（157.16±3.86） pg/ml] and IL -6 [ （67.76± 15.51） pg/ml vs 2.76 ±0.47） pg/ml] ,P 〈0.05. IL - 10 and IL - 12 levels in both groups had no significant difference. Conclusion：NETs can significantly increase proinflammationtory eytokines＇ secretion of monocytes, including IL - 1β, IFN - α, TNF -α, MCP - 1 and IL - 6.