microRNA-509-3p对肝癌HepG2细胞增殖和侵袭能力的影响

Effects of microRNA-509-3p on proliferation and invasiveness of liver carcinoma HepG2 cells

目的:探讨微小RNA(miR)-509-3p对肝癌细胞增殖和侵袭能力的影响及其机制。方法:选取60例肝癌患者癌组织及癌旁组织,采用实时荧光定量PCR法检测miR-509-3p的表达。培养Hep G2细胞,分别转染miR-509-3p类似物和miR-509-3p抑制剂或X连锁凋亡抑制蛋白(XIAP)siRNA,以不进行任何处理的细胞为空白对照,利用CCK-8试剂及Transwell小室分别检测miR-509-3p表达对Hep G2细胞增殖和侵袭能力的影响。结果:miR-509-3p在肝癌组织中的表达低于癌旁组织(P<0.001)。miR-509-3p类似物组细胞增殖能力和细胞侵袭数均低于空白对照组(P均<0.05),miR-509-3p抑制剂组细胞侵袭数高于空白对照组(P<0.05),miR-509-3p过表达可抑制XIAP的表达(P<0.05)。结论:miR-509-3p表达对肝癌细胞的增殖和侵袭能力有抑制作用,其机制可能是miR-509-3p低表达促进XIAP上调从而促进肝癌细胞增殖、增加其侵袭能力。

Aim：To explore the role of microRNA（miR）-509-3p in proliferation and invasion of liver carcinoma cells.Methods： A total of 60 cases of liver carcinoma tissue and 60 cases of paracancerous tissue were selected,and the miR-509-3p expression was detected by real-time PCR.HepG2 cells were cultured and transfected with miR-509-3p mimic,miR-509-3p inhibitor or XIAP siRNA,and cells without any treatment were the control group.The cell proliferation activity was detected by CCK-8,and cell invasion was detected by Transwell cell migration assay.Results： The expression of miR-509-3p in liver carcinoma tissue was significantly lower than that in paracancerous tissue（P〈0.001）.Compared with the control group,the cell proliferation rate and the number of migration cells in miR-509-3p mimic group were significant lower（P〈0.05）,while those in miR-509-3p inhibitor group were higher（P〈0.05）,and miR-509-3p overexpression contributed to the inhibition of XIAP expression（P〈0.05）.Conclusion： miR-509-3p plays an important role in the development and progression of liver carcinoma,and its downregulation of miR-509-3p may be closely associated with the high expression of XIAP.