MicroRNA-133a在5-氟尿嘧啶促进胃癌细胞BGC823及SGC7901细胞凋亡中的功能研究

Effect of microRNA-133a on 5-fluorouracil-induced apoptosis of BGC823 and SGC7901 cells

[目的]研究microRNA-133a(miR-133a)在5-氟尿嘧啶(5-Fu)诱导胃癌细胞BGC823及SGC7901凋亡中的作用。[方法]用荧光定量PCR检测经5-Fu(IC50值)处理过胃癌细胞miR-133a的表达;构建过表达miR-133a的胃癌细胞株并行细胞增殖、细胞流式验证miR-133a的生物学功能;5-Fu分别处理过表达及敲低miR-133a的胃癌细胞株后,行细胞增殖、细胞流式、Western Blot观察细胞生长及凋亡情况。[结果]5-Fu诱导BGC823及SGC7901内源性miR-133a的表达(>50%,P<0.0001,24h),miR-133a促进胃癌细胞凋亡,敲低miR-133a可以抑制5-Fu对胃癌细胞的促凋亡作用。[结论]5-Fu通过诱导胃癌细胞BGC823及SGC7901内源性miR-133a的表达,促进胃癌细胞凋亡。

[Objective] To study the effect of microRNA-133a （miR-133a） on 5-fluorouracil （5 Fu）-in- duced apoptosis of gastric cancer BGC823 and SGC7901 cells.[Methods]The expression of miR-133a in gas- tric cancer cells treated with 5-Fu was detected by polymerase chain reaction（PCR）;the biological function of miR-133a was confirmed by cell proliferation and cell flow assay. Over expression and knockdown of miR 133a gastric cancer cell lines were respectively tread with 5-Fu,and then cell proliferation,cell flow, Western Blot were designed to observe the growth and apoptosis of them. FResults]5-Fu induced BGC823 and SGC7901 endogenous miR 133a expression（〉50% ,P〈0. 0001,24 h）;miR-133a could promote the ap- optosis of gastric cancer cells;knockdown of miR 133a could inhibit the proapoptotic effect of 5 Fu on gas- tric cancer cells. [Conclusion]5-Fu promotes the apoptosis of gastric cancer cells by inducing the expression of endogenous miR-133a in BGC823 and SGC7901 cells.