摘要
目的探讨SHP-2蛋白在结直肠癌中的表达情况及其与病理特征的关系.方法采用免疫组织化学法和Western blot方法检测SHP-2蛋白在人结直肠癌组织中的表达情况,分析其与患者临床病理因素的关系.结果结直肠癌组织中S H P-2阳性表达率为25.6%(43/168),与正常结直肠组织比较,差异有统计学意义(P<0.05).结直肠癌组织中SHP-2的蛋白水平为0.2396±0.0655,与配对正常结直肠组织比较(0.7665±0.1133),差异有统计学意义(P<0.0001).SHP-2蛋白的低表达与分化程度和淋巴结转移有关,与性别、年龄、浸润程度、远处转移、TNM分期无关.结论SHP-2可能在结直肠癌的发生发展过程中起抑制作用,并可能成为潜在的治疗靶点.
AIM To detect the expression of SHP-2 protein in colorectal carcinoma（CRC） and to analyze its relationship with clinical characteristics.METHODS The expression of SHP-2 in CRC specimens was detected by immunohistochemisty and Western blot, and the association of CRC expression with clinicopathologic parameters of CRC patients was statistically analyzed.RESULTS The rate of positive expression of SHP-2 was significantly lower in CRC（25.6%） than in normal colorectal tissues（P 0.05）. SHP-2protein expression level in CRC（0.2396 ±0.0655） was significantly lower than that in paired normal colorectal tissues（0.7665 ±0.1133, P 0.0001）. The low expression of SHP-2 in CRC tissues was correlated with poor differentiation and lymph node metastasis, but not with gender, age, invasion depth, distant metastasis or TNM stage.CONCLUSION SHP-2 may be a tumor suppressor in CRC, and it may be used as a potential therapeutic target for this malignancy.
出处
《世界华人消化杂志》
CAS
2017年第22期2039-2044,共6页
World Chinese Journal of Digestology
基金
湖北省自然科学基金资助项目
No.2014CFB434
湖北省卫生计生科研基金资助项目
No.WJ2015MB080~~
