复星长征血清5'-核苷酸酶试剂在生化检验中的应用

Application of Fosun Long March serum 5 '-nucleotidase reagent to the determination of biochemical detection

目的:评价5'-核苷酸酶试剂(速率法)在自动化检测血清或血浆中5'-核苷酸酶活性的应用。方法:采用日立7180全自动生化分析仪,通过重复性、线性试验测定血清或血浆中5'-核苷酸酶活性,进行方法学评价并与美国Diazyme 5'-核苷酸酶试剂(速率法)比较。结果:两个水平质控中酶活性的变异系数(CV)分别为2.83%和1.96%,在5~200 U/L酶活性范围内,线性良好。两种试剂测定血清5'-核苷酸酶活性的相关系数较好,相关系数r为0.995。结论:自主研发的5'-核苷酸酶试剂盒操作简便、快速、准确、稳定,结果准确,可满足大批量临床样本的常规和自动化分析,是一种值得推广的方法。

Objective： To evaluate the application of serum 5＇-nucleotidase reagent to the conventional and automatic determination of 5 ＇-nucleotidase（ 5 ＇-NT） activity. Methods： Serum 5 ＇-NT activities were determined with HITACHI 7180 automatic biochemical analyzer through repeatability and linearity tests,the evaluation of methodology was carried out and the results were compared with those determined by Diazyme Laboratories in the United States. Results： The coefficients of variation（ CV） of the enzyme activity at two levels of quality control were 2.83% and 1.96% respectively. The determination of enzyme activity showed a good linearity over the range of 5 to 200 U/L. There was a good correlation between two reagents（ r = 0.995）. Conclusion： Independent 5＇-nucleotidase reagent made by our company can provide a simple,quick,accurate and stable method for the determination of serum 5＇-NT activity. It can meet the requirement of the conventional and automated analysis of mass clinical samples,which can be promoted.