野茼蒿对UC大鼠血清黏附分子表达的影响

Effect of Wild Chrysanthemum on Expression of Serum Adhesion Molecules in UC Rats

目的:通过研究野茼蒿对溃疡性结肠炎大鼠血清相关黏附分子表达的影响,探讨其对溃疡性结肠炎的疗效。方法:选择2,4,6-三硝基苯磺酸(TNBS)与乙醇混合液诱导的溃疡性结肠炎大鼠为研究对象,把UC模型大鼠分为:空白对照组、模型组,野茼蒿低、中、高剂量组,阳性对照组,每组各12只。每天给予各组不同药物处理。于给药21d后对大鼠结肠组织进行CMDI和HS评分,并用ELISA法检测血清黏附分子CD62P、PSGL-1、CD44、CD54的含量,比较各组间指标差异。结果:与空白组相比,模型组CMDI评分和HS评分以及血清中黏附分子CD62P、PSGL-1、CD44、CD54明显升高(P<0.01或P<0.05);与模型组比较,低、中、高剂量组大鼠上述两个评分以及黏附分子含量一定程度上降低(P<0.01或P<0.05);与模型组相比,SASP组大鼠血清述两个评分以及黏附分子的含量明显降低(P<0.01或P<0.05)。结论:野茼蒿冻干粉对UC大鼠有一定的治疗作用,其机制可能与影响CD62P、PSGL-1、CD44、CD54的表达有关。

Objective： To investigate the effect of Wild Chrysanthemum on the expression of serum-related adhesion molecules in rats with ulcerative colitis and to explore its effect on ulcerative colitis.Method： The rats of ulcerative colitis induced by 2, 4, 6-trinitrobenzene sulfonic acid （ TNBS ） and ethanol were selected as the study subjects.The UC rats were divided into six groups： blank group, model group, wild chrysanthemum Low, medium and high dose group, positive control group, 12 cases in each group.Each group was given a different medication every day.At 21 days after administration, CMDI score and HS score were performed on rat colon tissue. The levels of CD62P, PSGL-1, CD44 and CD54 in serum were measured by ELISA.The differences of six groups were compared and analyzed statistically.Result： Compared with the blank group, the CMDI scores and HS scores and the levels of CD62P, PSGL-1, CD44 and CD54 in the serum of the model group were significantly increased （ P〈0.01 or P〈0.05 ） ; compared with the model group, the two scores and the levels of adhesion molecules in serum of the low, medium and high dose rats groups were significantly lower （ P〈0.01 or P〈0.05 ） ; compared with the model group, the above two scores and the content of adhesion molecules in serum of SASP group were significantly decreased （ P〈0.01 or P〈0.05 ） .Conclusion： The freeze-dried powder of Wild Chrysanthemum has a certain therapeutic effect on the clinical symptoms of UC rats.The mechanism may be related to the expression of CD62P, PSGL-1, CD44 and CD54.