乙型肝炎患者T细胞亚群、IL-32水平的变化及其临床意义分析

Changes of T-cell Subsets and IL-32 in Patients with Hepatitis B and Its Clinical Significance

目的:对不同程度乙型肝炎患者血液中的T细胞亚群、IL-32水平进行测定,比较其变化情况并探讨其对血清免疫细胞的影响。方法:选取2016年3-12月来本院就诊的120例不同程度乙型肝炎患者,同期40例健康体检的志愿者作为对照组,采用流式细胞仪对T细胞亚群进行检测,酶联免疫吸附试验测定IL-32细胞因子的水平,分别比较不同组别的变化情况。结果:乙型肝炎患者血液中CD4^+ T细胞的百分比及CD4^+/CD8^+比值均低于对照组,而CD8^+ T细胞亚群的百分比高于对照健康组,差异均有统计学意义(P<0.05)。不同程度乙型肝炎组血清中IL-32的水平均高于对照组,差异均有统计学意义(P<0.05)。结论:乙型肝炎患者细胞免疫功能呈现紊乱状态,IL-32的水平明显增高可能是参与免疫应答,T细胞亚群比例异常与肝脏的炎症损伤程度具有相关性。

Objective： To study the changes of T cell subsets and IL-32 levels in blood of different degrees hepatitis B patients, to compare and explore their effect on serum immune cells.Method： From March to December 2016, 120 different degrees hepatitis B patients who were treated in our hospital were selected as observation group, g0 healthy volunteers were selected as the control group.Test T cells subsets by flow cytometry instrument, and levels of IL-32 cytokines were measured by enzyme-linked immunosorbent assay （ ELISA ）, and the changes of different groups were compared.Result： The percentage of CD4^＋T cells and CD4^＋/CD8^＋ ratio in the blood of patients with hepatitis B were lower than those of control group, while the percentage of CD8^＋ T ceils in the blood of patiensts with hepatitis B was higher than that of control group, the differences were statistically significant （ P〈0.05 ） ; the serum IL-32 levels of different degrees hepatitis B patients were higher than those in cases of control group, the differences were statistic significant （ P〈0.05 ） .Conclusion： The cellular immune function of hepatitis B virus patients is in a disorder state, the increase of IL-32 levels may be related to the severity of inflammatory response, and the degree of T cell subsets ratio anomalies associated with inflammation damage degree of liver.