气阴两虚夹瘀型消渴目病血清细胞因子表达变化的研究

Study on the changes of serum cytokine expression in patients with diabetic retinopathy(deficiency of Qi and Yin with blood stasis syndrome)

目的:研究气阴两虚夹瘀型糖尿病视网膜病变(DR)患者血清血管内皮生长因子(VEGF)、色素上皮细胞衍生因子(PEDF)含量的变化。方法:从福建中医药大学附属人民医院收集随机健康体检者30例为正常组、消渴病患者30例为DM组、气阴两虚夹瘀型消渴目病患者30例为DR组。采用酶联免疫吸附(ELISA)法检测正常组、DM组和DR组血清VEGF、PEDF含量,比较3组间血清VEGF、PEDF含量差异。结果:正常组、DM组和DR组血清VEGF含量逐渐上升,3组间比较,差异有统计学意义(Z=35.84,P<0.01);正常组、DM组和DR组血清PEDF含量逐渐下降,3组间比较,差异有统计学意义(Z=16.29,P<0.01)。结论:血清VEGF、PEDF含量与消渴目病发生、发展有一定关系,血清VEGF含量随着消渴目病病情发展逐渐上升,血清PEDF含量随着消渴目病病情发展逐渐下降,二者可作为消渴目病发生、发展评估的指标。

Objective：To study the expression of VEGF and PEDF in patients with diabetic retinopathy（DR）. Method：30normal volunteers（normal group）in medical center,30 cases of patients with diabetesbut without DR（DM group）in endocrinology,30 cases of patients with DR in ophthalmology（DR group）were collected. The serum VEGF and PEDF levels were detected by enzyme-linked immunosorbent assay（ELISA）in three groups. Result：Serum VEGF levels were risen gradually and PEDF levels were decreased of normal group,DM group and DR group,the difference among three groups was significant（P0.01）. Conclusion：Serum VEGF and PEDF levels may be related to occurrence and development of DR. With progression of DR,VEGF level was increased,but PEDF was decreased gradually. Both VEGF and PEDF can be used as indicators to assess development of DR.