燕窝对再生障碍性贫血小鼠血常规及继发流感病毒感染的影响

Effect of edible bird＇ s nest on hemogram and secondary influenza virus infection of mice with aplastic anemia

目的探讨食用燕窝对再生障碍性贫血（再障）继发流感病毒感染的保护作用。方法食用燕窝的药理测定。体外实验采用血液凝集抑制试验和细胞感染中和试验，体内实验采用再障小鼠动物模型和病毒感染模型，观察燕窝酶解物对再障小鼠血常规的影响，判断食用燕窝的生物药效。结果经胰蛋白酶水解后的燕窝酶解物N1-Pan和N2-Pan的血凝抑制活性分别为4μg／mL和125μg／mL，具有较强的抑制血凝的活性。N1-Pan对流感病毒有很强的抑制作用（IC50为40μg／mL）。小鼠喂养实验发现，无病毒燕窝喂养组第20天血常规比第10天有明显改善（提升10％-20％），组内比较差异有统计学意义（确切概率法，P〈0．05）；燕窝喂养的接种流感病毒再障小鼠较未喂养燕窝的再障小鼠外周血常规和体重有明显改善（确切概率法，P〈0．05），存活率也显著高于后者（4／8：7／8，确切概率法，P〈0．05）。结论N1-Pan能明显改善再障小鼠的外周血常规和体重，提高流感病毒感染再障小鼠的存活率。

Objective To study of the protective effect of edible bird＇s nest on aplastic anemia （AA） following secondary infection of influenza virus. Methods Pharmacology of edible bird＇ s nest was determined by in vitro and in vivo test. The hemagglutination inhibition test and the neutralization test for infected cells were undertaken in vitro, while the AA mice animal model and virus infection model were adopted in vivo. By in vitro and in vivo experiments, the bird＇s nest hydrolysates effect on AA mouse blood was observed, in order to determine the bioavailability of edible bird＇s nest. Results The hemagglutination inhibition level mouse effect of edible bird＇s nest zymolyte N1-Pan and N2-Pan after trypsin hydrolysis were 4 μg/mL and 125 μg/mL, which had relatively high activity of inhibition to hemagglutination. N1-Pan had strong neutralizing capacity against influenza virus （IC50=40 μg/mL）. In animal feeding experiments, the hemogram of AA mice on the 20th day after feeding N1- Pan for 10 days was obviously better than that on the 10th day（improving 10% to 20%）, of which the difference had statistical significance （Fisher＇s exact test, P〈0.05）. AA mice with only influenza virus inoculation mouse on the 20 th day that N1-Pan for 10 days only after feeding NI-Pan had improved peripheral hemogram and weight the than AA mice with influenza virus inoculation （Fisher＇s exact test,P〈0.05）, and the survival rate of the former was nmch higher than the latter （4/8 vs 7/8,Fisher＇s exact test ,P〈0.05）. Conclusions N1-Pan can significantly improve the AA mice peripheral hemogram and body weight, and increase survival rate of AA mice infected withinfluenza virus.