四球茶转录组SSR位点信息分析

Analysis of SSR information in Camellia tetracocca Zhang transcriptome

对基于高通量测序拼接获得的99 741条四球茶嫩叶Unigenes进行了SSR(simple sequence repeats)位点分析,共揭示了23 732个SSR位点,分布于18 552条Unigenes中,SSR发生频率为23.79%;含有SSR位点的Unigenes的总长度为226 188 bp,SSR位点间的平均距离为1/2.07 kb,重复长度平均长度为9.53 bp。在四球茶转录组SSR中,二核苷酸重复和三核苷酸重复是主要的重复类型,分别占总SSRs的47.53%和25.92%;在181种重复基元类型中,优势重复基元分别是AG/CT、A/T、ACC/GGT,分别占总SSRs的41.38%、22.10%和6.49%,共占总SSRs的比例达69.97%。

In this study, a total of 99 741 unigenes were de novo assembled based on the bud transcriptome se- quencing of Camellia tetracocca Zhang. Totally, 23 732 SSRs were detected in 18 552 unigenes, accounting for 23.79% of all the unigenes. The total length of unigenes containing SSR loci was 226 158 bp. The mean distance between SSRs was 1/2.07 kb and the mean length of the repeat motif was 9.53 bp. Di-nucleotide repeats were the most abundant followed by tri-nucleotide repeats, making up about 47.53% and 25.92% of the total SSRs, respec- tively. Among the 181 repeat motifs, AG/CT, A/T and ACC/GGT were the most frequent motifs in di-, mono- and tri-nucleotide repeats, accounted for 41.38%, 22.10% and 6.49%, respectively, and which totally accounted for 69.97% of the total SSRs. These results will provide abundant sequences for SSR markers development on Camellia tetracocca Chang.