双孢蘑菇新菌株蛋白质的营养评价与分析

Assessment and Analysis of Protein Nutrition in New Strains of Agaricus bisporus

本研究测定了双孢蘑菇亲本FA15与突变株FA15-5、FA15-6子实体的氨基酸含量,并利用非生物学指标,以鸡蛋蛋白为标准蛋白,以WHO/FAO必需氨基酸参考模式为评价标准,对双孢蘑菇子实体的蛋白质营养价值进行了全面评价。结果表明:亲本与两个突变株的氨基酸种类齐全,必需氨基酸占氨基酸总量的百分比分别为36.99%、39.31%、36.53%。亲本和突变株的限制性氨基酸均为异亮氨酸。氨基酸评分(AAS)显示,FA15-5、FA15-6比FA15分别低31.71%高25.00%。突变株FA15-6的必需氨基酸指数(EAAI)值较FA15高39.97%,生物价(BV)较FA15高54.73%,营养指数(NI)值较FA15高40.00%。因此,在营养价值方面,FA15-6是优于亲本的良好突变菌株。

Through the determination of amino acid content in sporocarps,the parent Agaricus bisporus strain FA15 and its mutant strains FA15-5 and FA15-6 were comprehensively assessmented in protein nutritive values using the non-biological indicants with the egg protein as standard protein and the essential amino acid reference pattern of WHO/FAO as evaluation criterion. The results showed that the kinds of amino acids in the parent and the two mutant strains were complete,and their essential amino acids accounted for36. 99%,39. 31% and 36. 53% of the total amino acids,respectively. Their restrictive amino acid was isoleucine. The amino acid scores of FA15-5 and FA15-6 were 31. 71% lower and 25. 00% higher than that of FA15 respectively. The EAAI value,biological value and nutrition index of mutant strain FA15-6 were39. 97%,54. 73% and 40. 00% higher than those of the parent strain FA15 respectively. In conclusion,FA15-6 was a better mutant strain with better nutritional value than the parent strain FA15.